Objective: To study the dead number of spinal cord anterior horn motor neurons following sciatic nerve injury in adult rats.

Methods The number of spinal motoneurons was counted at different culturing time(such as 0;1;3;5;7;9days)under microscope at250×.

Methods:GSH and L-NAME at different concentration were added to spinal motoneurons in vitro.

Part I The culture and identification of spinal motoneurons in vitroObjective: To explore the dissociation, the purity and the culturing method in vitro of spinal motoneurons and to find out an effective way to identify this kind of cells.

Identification and culture of rat embryonic spinal motor neurons;

Effect of glia cell line-derived neurotrophic factor on cultured spinal motor neurons from embryonic rat;

To find out the rules of changes in excitability of antagonist spinal motor neurons when the muscle fatigue of agonist occurs, ankle joint dorsal flexion form was applied to investigate the soleus muscle H wave under the state of tibialis anterior muscle fatigue.

To investigate molecular mechanisms of AMAN, proteomics was applicated to evaluate the effect of serum from AMAN patients on spinal motor neurons of rat in this study.