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1)  single cell gel electrophoresis
单细胞电泳
2)  single cell gel electrophoresis
单细胞凝胶电泳
1.
Investigation on Accordance of DNA Double-strand Break of Blood Between in Vivo and in Vitro Irradiation Using Single Cell Gel Electrophoresis;
单细胞凝胶电泳技术对离体和整体照射致细胞DNA断裂的一致性研究
2.
Relationship between Nuclear DNA Degradation of the Rat’s Spleen Cells and Postmortem Interval Assayed by Single Cell Gel Electrophoresis;
应用单细胞凝胶电泳检测大鼠死后脾细胞核DNA降解与死亡时间的关系
3.
Research on K562 DNA damage assayed by single cell gel electrophoresis (SCGE);
应用单细胞凝胶电泳技术检测K562细胞DNA损伤的研究
3)  single cell gel electrophoresis(SCGE)
单细胞凝胶电泳
1.
DNA damage in somatic cells was determined by single cell gel electrophoresis(SCGE),and the relationship between DNA damage and HQ dosage was analyzed.
0 m g/m3)的氢醌染毒2周,用单细胞凝胶电泳技术(SCGE)检测氢醌染毒后的小鼠肝、肾、脾、骨髓、胸腺和外周血淋巴细胞的DNA损伤情况,分析肺外组织DNA损伤与氢醌剂量间的关系。
2.
DNA damage were analyzed by single cell gel electrophoresis(SCGE),O~6-MeG was analyzed by high performance capi.
干预结束后,采用单细胞凝胶电泳法检测和评价淋巴细胞DNA自发损伤和H2O2诱导的氧化损伤;留取尿液用毛细管电泳法检测大鼠尿中O6-甲基鸟嘌呤(O6-methyl guanine,O6-MeG)含量。
3.
5 mg/kg once a week for 8 weeks, DNA damage of peripheral lymphocytes and bone marrow cells were examined by single cell gel electrophoresis(SCGE) .
5 mg/kg腹腔注射,每周1次,共8周;用单细胞凝胶电泳方法检测其外周血淋巴细胞及骨髓细胞DNA的损伤情况。
4)  SCGE
单细胞凝胶电泳
1.
Detection of DNA Damage in Human & Mouse Sperm by Modified SCGE;
采用单细胞凝胶电泳技术检测断裂剂对精子DNA损伤
2.
Necropsy was carried at 24 h, DNA damage of peripheral blood lymphocytes was measured by the single-cell gel electrophoresis( SCGE,comet assay).
一次性腹腔注射给药,24h后,用单细胞凝胶电泳技术检测3种制剂对小鼠外周血淋巴细胞DNA的损伤作用。
3.
5mg/L level on hepatopancreas of loach Misgurnus anguillicaudatus by SCGE for 1—35 days exposure.
采用单细胞凝胶电泳技术(SCGE),研究重金属Cd2+、Pb2+、Zn2+在不同暴露时间(1—35d)、单一重金属离子不同暴露浓度(0。
5)  Single-cell gel electrophoresis
单细胞凝胶电泳
1.
Single-cell gel electrophoresis(SCGE)was used to detect the DNA damage induced by sodium nitrite in the sertoli cells.
[方法]采用冷胰酶消化法分离培养大鼠睾丸支持细胞,采用单细胞凝胶电泳法检测亚硝酸钠对大鼠睾丸支持细胞DNA的损伤作用。
2.
2, 2, 20, 100, 200 μmol/L for 1 and 2 h, DNA damage of spleen lymphocytes were measured by the single-cell gel electrophoresis (SCGE).
方法:采用单细胞凝胶电泳法(SCGE)分别测定BALB/c小鼠脾淋巴细胞体外染毒0。
3.
BACKGROUND & AIM: The effect of nonylphenol(NP) on DNA damage in oyster hemolymph cells was measured by single-cell gel electrophoresis assay in vivo.
背景与目的:采用单细胞凝胶电泳技术研究壬基酚(nonylphenol,NP)对牡蛎血淋巴细胞DNA的损伤作用。
6)  Comet assay
单细胞凝胶电泳
1.
Improvements on an Image Analysis System for Comet Assay;
单细胞凝胶电泳图像分析系统改进
2.
In order to explore and compare the DNA damage effects on cells induced by nanosized MnO2 and normal MnO2 powder, Hela cells were exposed with different concentrations of these two powders(0, 100, 200, 400μg·mL-1),The comet assay was applied to measure the DNA damage.
为探讨纳米MnO2与常规MnO2粉末对细胞DNA损伤作用的差别,采用不同浓度的纳米MnO2与常规MnO2粉末(0、100、200、400μg·mL-1)对Hela细胞进行染毒,应用单细胞凝胶电泳(彗星实验)检测Hela细胞的损伤效应。
3.
Method:The effects of sodium fluoride on DNA damage and repair in isolated male mice testicular cells were observed with single cell gel electrophoresis test(Comet assay).
方法 :应用单细胞凝胶电泳试验 (SCGE)研究了氟化钠对离体小鼠睾丸细胞的DNA损伤与修复效应。
补充资料:单细胞克隆技术
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性质:平板培养是为了分离和获得单细胞无性系并对不同的无性系进行研究,揭示它们在生理、生化和遗传上的差异而设计的一种单细胞培养技术,也称单细胞克隆技术。由于它具有筛选效率高、筛选量大、操作简单便等优点,因而被广泛地用于遗传变异、细胞分裂分化、细胞次生代谢物合成等项研究。这项技术是1960年Bergmann首创的,操作时将单细胞悬浮液与30~35℃呈融化状态的琼脂均匀混合,然后注入培养皿中成一薄层,琼脂冷却固化后,均匀分布的细胞就在其中成长,由于薄薄的一层固化琼脂呈平板状,故称它为平板培养。

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