The PCR primers design and identification for the detection of beer spoilage lactic acid bacteria;

Amplification efficiency test for primers in real-time PCR reactions;

Screening of Random Primer and Optimizing of Reaction System for Arbitrarily Primed Polymerase Chain Reaction on 8 Sorts of Bacteria;

3 -terminus shifted bases degeneracy primers increasing sensitivity of polymerase chain reaction;

Optimization for SRAP-PCR system in Cynodon dactylon and selection of primers;

Selection of primers and establishment of SSR-PCR reaction system on Pinus tabulaeformis Carr.;

Detecting enterovirus by reverse transcription nested-polymerase chain reaction established with universal primers;

A rapid quantitative polymerase chain reaction(QPCR) analysis method with universal primers was developed to detect cell densities of the enteric pathogenic bacteria from 5 surface water of Xi'an City for 4 months continuously.

In order to investigate the application values of polymerase chain reaction(PCR) technique in detection of pathogenic bacteria in surface water,the universal primers were designed and synthesized according to the high conversation of 16S rRNA gene.

The study is to assess the effects of the important primer parameters such as GC contents,Tm and secondary structure,which were showed by primer designe software Primer 5.

Due to the lack of polyadenylation in prokaryotic mRNA, the primer design of prokaryotic DDRT-PCR is different from eukaryocyte.

A primer design method was developed for detection of point mutation in rice.

Based on the sequence of internal transcribed spacer regions(ITS)of the ribosomal gene,a couple of specific primers for P sojae was synthesized,and a single band of PCR product about 330 bp was amplified from the crashed sample of zoospore or oospores carried in the soil or the diseased soybean tissues.