1) Enhanced RACE PCR
增强RACE
1.
Enhanced RACE PCR was used to clone the full-length cDNA of RS1,including enrichment of the target gene through biotin-labeled probe for magnetic bead pur.
方法 RT PCR方法对RS1基因进行表达谱分析 ,找到表达丰度较高的组织提取总RNA作为模板 ,应用增强RACE技术即结合生物素探针富集靶cDNA的方法克隆RS1的cDNA末端。
3) Rapid amplification of cDNA ends
RACE
1.
The porcine CD8α full-length cDNA was amplified and cloned from total RNA of lymphocytes by RT-PCR and RACE(rapid amplification of cDNA ends).
应用RT-PCR和RACE技术从猪胸腺细胞总RNA中扩增克隆了猪CD8α的全长cDNA基因。
2.
By the methods of RT-PCR and rapid amplification of cDNA ends(RACE) we isolated a HSP70 gene(CsHSP70) from Capparis spinosa,a specific resistant plant in arid zone.
以干旱区特有的抗逆性植物刺山柑为材料,利用RT-PCR结合cDNA末端快速扩增(rapid amplification ofcDNA ends,RACE)方法,克隆了一个HSP70基因,将该基因进行原核表达,通过对大肠杆菌进行温度胁迫实验,并计算存活率来验证该基因对细胞的保护作用。
4) rapid amplification of cDNA ends(RACE)
cDNA末端快速扩增(RACE)
5) RACE (Rapid Amplification of cDNA End)
RACE(cDNA链末端的快速扩增)
6) 3 RACE
3'RACE
1.
3 RACE Amplification and Sequence Analysis of a Strain of Norovirus;
一株诺如病毒的3’RACE扩增及基因序列分析
2.
Objective:To amplify the 3 -cDNA end of phospholipase D(PLD)gene of Agropyron mongolicum Keng by 3 RACE technique and to analyze the sequence in the experiment,the 3 -cDNA end cloning establish foundation in order to obtain the cDNA sequence and research the function of PLD gene.
目的:采用3’RACE方法对蒙古冰草磷脂酶D(PLD)基因3’端全序列进行了快速扩增和序列分析,为得到全序列及研究该基因的功能奠定基础。
3.
The cDNA of DMrt1(DM-related transcription factor1)was amplified by 3 RACE-PCR(Rapid Amplification of cDNA Ends-Polymerase chain reaction)strategy using the total isolated RNA as template.
从奥利亚罗非鱼精巢提取总RNA,采用RT和3’RACE(rapid amplification of cDNA ends)法分离和测定了奥利亚罗非鱼DMRT1(DM-related transcription factor1)cDNA的3’序列,扩增得到1086bp的片段,阅读框共编码254个氨基酸,序列分析结果表明,奥利亚罗非鱼DMRT1基因的氨基酸序列与尼罗罗非鱼DMRT1基因的同源性为99%,与黑鲷、黄鳝、新月鱼、虹鳟、青鳉鱼等动物的DMRT1cDNA和尼罗罗非鱼、奥利亚罗非鱼等动物的DMO(DM-domain gene in Ovary)cDNA的同源性分别为:80%、78%、73%、62%、41%、26%、26%。
补充资料：“质子-电子偶极-偶极”质子弛豫增强
“质子-电子偶极-偶极”质子弛豫增强
物理学术语。原子核外层中不成对的电子质量小，但磁动性很强，可使局部磁场波动增强，促使氢质子弛豫加快，从而使T1和T2缩短，这种效应即为PEDDPRE。过渡元素和镧系元素大部分在d和f轨道有多个不成对电子，所以其离子往往具有PEDDPRE，可用来作顺磁性对比剂，如钆(Gd)。Gd在外层有7个不成对电子，具有很强的顺磁性。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条