Methods: L type calcium channel α_1D subunit specific gene of human odontoblasts was cloned from human odontoblast-like cell line by RT-PCR, target fragment was inserted into pUC18 vector and sequenced.

Construction and identification of proeukaryotic expression plasmid of L type calcium channel α_1 D subunit specific gene of human odontoblast;

Isolation and identification of human odontoblast cells;

Effects and molecular mechanism of nicotine on odontoblasts;

Research progress of odontoblast s specific molecular markers;

Age-related changes in expression of HSP70 in odontoblasts;

Induced differentiation of human embryonic ectomesenchymal stem cells of facial processes to odontoblast-like cells in three-dimensional culture system;

Mineralization of induced odontoblast-like cells from facial ectomesenchymal cells in vivo and in vitro;

Concentration optimization of dentin non-collagenous protein on differentiation of ectomesenchymal cells of mandibular process to odontoblast-like cells

Observation of odontoblasts and dentin in neonatal mice with light and electronic microscopy;

In situ Hybridization Analysis of USF1mRNA Expression in Odontoblasts;

Method:The expression of NOSs in the odontoblasts and pulp cells was detected by immuno- histochemistry statining on crysection after the cavity prepartion on the mesial surface of the maxillary first molars of rats.