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1)  Taq man
SYBR
1.
The HBVDNA in culture media were prepared with three methods for isolation and amplified by Taq man and SYBR real-time quatitiative PCR.
方法分别采用3种方法抽提HBVDNA,抽提物分别采用Taqman荧光定量、SYBR荧光定量PCR技术检测其滴度。
2)  SYBR Green I
SYBR GreenI
1.
Standard curve was established using a series dilution of quantified plasmids to measure EZH2 using SYBR Green I real-time fluorescent polymerase chain reaction and the characteristic of specific EZH2 amplicon was analysed by melti.
目的:建立SYBR GreenI实时荧光PCR定量检测人类EZH2基因的方法。
2.
According to the avian reticuloendotheliosis virus(REV) LTR sequence available in GenBank,a pair of specific primers were designed which target to the conserved region of LTR,and SYBR Green I-based real-time PCR was developed.
根据禽网状内皮组织增生症病毒(REV)长末端重复序列LTR基因保守区域设计并合成一对引物,建立基于SYBR GreenI模式的实时荧光PCR方法(Real-timePCR)。
3)  SYBR green Ⅰ
SYBR greenⅠ
1.
A rapid and sensitive Real-time PCR assay coupled with SYBR Green Ⅰchemistry was developed for the quantitative detection of Turbot reddish body iridovirus (TRBIV) isolated from farmed turbot (Scophthalmus maximus).
研究利用TRBIV主要衣壳蛋白基因序列设计的1对引物,结合内嵌式核酸染料SYBR GreenⅠ,建立了TRBIV特异的Real-timePCR检测方法。
4)  SYBR dye
SYBR染料
5)  SYBR GreenⅠ
SYBR Green Ⅰ
1.
Establishment of SYBR GreenⅠReal-Time Fluorescent Quantitative PCR for Detection of Duck Plague Virus;
SYBR Green Ⅰ实时定量PCR快速检测鸭瘟病毒的研究
6)  SYBR Green I real-time PCR
SYBR~ GreenI实时PCR
补充资料:Malachite Green Crystals
分子式:2(C23H25N2)·3C2H2O4
分子量:929.05
CAS号:569-64-2

性质:绿色闪光结晶,溶于冷水和热水呈蓝绿色,易溶于酒精,也呈蓝绿色。遇浓硫酸呈黄色,稀释后呈暗橙色;其水溶液加氢氧化钠形成微带绿光的白色沉淀。属三苯甲烷系碱性染料。

制备方法:由N,N-二甲基苯胺与苯甲醛在硫酸存在下进行缩合,再用二氧化铅和盐酸进行氧化,然后用硫酸钠脱铅,用碳酸钠中和,然后经草酸溶解、结晶、过滤干燥即得成品。原料消耗(kg/t)N,N-二甲苯胺 780 盐酸(31%) 1250尿素 6 氢氧化钠(100%) 350苯甲醛 340 次氯酸钠 4820乙酸铅(98%) 215 元明粉(98%) 570草酸(98%) 520 精盐 1900纯碱(98%) 290 乳化剂OP 0.7硫酸(100%) 305

用途:用于麻、蚕丝、腈纶织物和草制品以及竹、木等的染色。还可制成各种色淀及颜料。

说明:补充资料仅用于学习参考,请勿用于其它任何用途。
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