The study on enucleation of somatic cell nuclear transfer in Kunming mouse;

The major object of this paper is to isolate and culture Kunming mouse EG cells as well as to identify the EG cells from various aspects.

Effect on isolation and culture of Kunming white mouse ES cells with media conditioned of rat cardiomyocyte;

The present experiments were conducted to evaluate the effect of gonadotropins on Kunming white mouse oocyte maturation and the relationship between cumulus cell expansion and oocyte maturation in vitro .

Methods: The spermatogonial stem cell allotransplantation was performed, without the use of immune inhibitor, in KM mice of similar gene types, and the spermatogenesis in recipients testes was evaluated.

Since 1998, after the first successful mouse somatic cloning, the mouse somatic cell nucleus transplant technology was perfect gradually, many strain s mice have all been successfu in the somatic cell nucleus transplant, obtained the clone descendant, but KM mouse has not been the successful report actually.

[Method]Kunming mice were injected through abdominal cavity one time with different dosage of PHA,observing their acute toxicity status and mortality situation.