Cloning and prokaryotic expression of cathepsin D like aspartic protease gene of Clonorchis sinensis and analysis of immunogenicity of the recombinant protein;

The yapsins are a novel class of glycosylphosphatidyl inositol(GPI)-linked aspartic proteases in fungus.

Objective With the help of the bioinformatics to predict the deduced protein\'s structure and function,we identified cathepsin D-like aspartic protease from the full length cDNA plasmid library of Clonorchis sinensis in order to instruct the experiment.

Research advancement on aspartic proteinase;

Of its fermentation broth, there were properties that resembles as in an acidic and heat-stabled to that of the aspartic proteinase.

Objective: Investigating the expression of Caspase-3 mRNA by reverse transcription polymerasechain reaction(RT-PCR) in the normal and preeclampsia placentas in order to evaluate the role of Caspase-3 in preeclampsia.

Eventually resistant tumor cells manifest as inhibition of the activation of Caspase-3,and reduction of apoptosis.

To investigate the effect of hydrogen peroxide(H2O2)on the expression of caspase-3 and the changes of ultra-structures in protoscolex cells of Echinococcus granulosus in vitro,the protoscolex cells were cultured in vitro with special RMPI-1640 medium in which glutamine was added.