The F1 and F2 of F gene of Newcastle disease virus strain Mukteswar were amplified with reverse transcription-polymerase chain reaction,the goal gene pieces and plasmid pGAPZαA were digested with KpnⅠand XbaⅠ,the digested pieces were linked with DNA ligase,E.

The F1 and F2 of F gene of Newcastle disease virus isolation JZ05 were amplified with reverse transcription-poly-merase chain reaction,the goal gene pieces and plasmid pGAPZαA were digested with KpnⅠand XbaⅠ,digested pieces were linked with DNA ligase,Eacterium coli DH5α were transformed by the linked outcomes.

Shuttle plasmid construction of Signature-Tagged Mutagenesis technology;

Construction and identification of shuttle plasmid expressing ICL-GFP fusion protein in Mycobacterium;

Construction,identification and expression of three kinds of shuttle plasmids of adenovirus expression vector of hepatitis C virus structure gene;

To construct and identify E coli-mycobacterium recombinant shuttle vector expressing secreted protein MPT64 of Mycobacterium tuberculosis.

BACKGROUND&AIM: Using a new SV40-based shuttle vector pSP189and African green monkey kidney cells(Vero E6cell line),we constituted a shuttle vector/mammalian cell system to detect mutagenesis in vitro induced by olaquindox.

A SV40 based shuttle vector pSP189 and African green monkey kidney cell line (Vero) was appied , which constitute a shuttle vector /mammalian cell system to detect mutagenesis in vitro induced by animal drugs olaqiundox and quinocetone.