1) Suicide plasmid
自杀质粒
2) recombination suicide vector
重组自杀性质粒
1.
Firstly, the recombination suicide vector with 320 bp-deleted crp gene and 720 bp-insert gfp gene was constructed and conjugated with C500.
首先构建含缺失320bp的crp基因并插入gfp基因的重组自杀性质粒,与C500接合转移,两步法筛选无抗性的crp-/gfp+缺失株,经PCR、测序鉴定和荧光显微镜观察证实在C500的基因组上成功缺失了crp基因并正确插入了gfp基因。
2.
Firstly, the recombination suicide vector with 320 bp-deleted crp (cAMP receptor protein) gene and sacB (sucrose-sensitive gene) gene was c.
首先构建含缺失320bp的crp(cAMP受体蛋白)基因与蔗糖敏感基因(sacB)的重组自杀性质粒,与C500接合转移,两步法筛选无抗性的△crp缺失株,用PCR证实基因组crp基因的缺失突变。
3) suicide vector pRE112
自杀性质粒pRE112
4) suicide vector pSUP202
自杀性质粒pSUP202
5) killer plasmid
嗜杀质粒
补充资料：PUC18质粒DNA
CAS: 9007-49-2
中文名称: PUC18质粒脱氧核糖核酸；PUC18质粒DNA
英文名称: Deoxyribonucleic-acid；Calf thymus DNA；Desoxyribose nucleic acid；DNA；Nucleic acids, deoxyribo；deoxyribonucleic ac.；Nucleic acids,deoxyribo
中文名称: PUC18质粒脱氧核糖核酸；PUC18质粒DNA
英文名称: Deoxyribonucleic-acid；Calf thymus DNA；Desoxyribose nucleic acid；DNA；Nucleic acids, deoxyribo；deoxyribonucleic ac.；Nucleic acids,deoxyribo
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条