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1)  Cleavage motif
酶切基序
2)  hFEN1
内切酶基因
1.
To increae the efficiency of HSA transgenic Mice,non-coding large DNA fragment?hFEN1 and the human HSA genes were introduced into fertiliged mice eggs by mixed microinjection to product the animal model of HSA transgenic mice.
将无基因的序列CIT987SK-384D8(30号)和结构特异的内切酶基因hFEN1穴36号雪与HSA的cDNA(26号)进行混合注射来制备转HSA小鼠动物模型,旨在提高转HSA基因效率。
3)  excisionase gene(xis)
切除酶基因(xis)
4)  Genetic enzyme cutting identification
基因酶切鉴定
5)  CAPS
酶切扩增多态性序列
1.
Both phenotypic selection and CAPS (Cleaved amplified polymorphic sequence) marker assistant selection were conducted in segregated population to determine whether the plant has elite agronomic traits and TT site in Wx genotype.
利用高产早稻品系4384和优质早稻品种舟903杂交,杂交后代与4384回交后连续自交,在分离世代利用Wx基因的酶切扩增多态性序列(CAPS)标记选择Wx基因型中具有TT位点的单株或株系,并在田间选择农艺性状。
6)  restriction enzyme digestion
酶切
1.
The isolated plasmid DNA was quantified by spectrophotometric measurement,and evaluated by the electrophoresis of plasmid DNA in an agarose gel,the restriction enzyme digestion,and the transfection into eukaryotic cells.
方法将含有目的质粒的菌株扩增后,运用改良后的碱裂解法进行质粒DNA小量提取,微量核酸仪测定提取质粒DNA的产量及纯度;采用琼脂糖凝胶电泳、内切酶酶切及质粒转染真核细胞鉴定提取质粒DNA的质量及转染效率。
补充资料:酒化酶、醇酶、发酵酶
分子式:
CAS号:

性质:又称酒化酶、醇酶、发酵酶。从酵母中分离而得的一系列酶。不耐热。它能催化酒精发酵反应,与糖酵解有关,如磷酸化酶、醛缩酶、葡萄糖磷酸变位酶、葡萄糖6-磷酸酶等。

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