1)  RNase P
核酶P
1.
Methods:The M1GS-T6 ribozyme was constructed by linking the catalytic RNA subunit of RNase P from Escherichia coli(M1 RNA) covalently to a guide sequence(GS) that is complementary to the mRNA coding sequence of UL97,which encode a phosphotransferase of HCMV.
方法:针对HCMV UL97 mRNA T6位点设计与之互补的引导序列(Guide Sequence,GS),将其共价结合至大肠杆菌核酶P催化亚基(M1 RNA)的3′末端,构建M1GS-T6核酶,并用其对UL97基因亚克隆片段转录产物进行体外靶向切割实验。
2.
Methods Using the pFL117 plasmid,which containing M1 gene of the RNase P from Escherichia coli as the template,DNA products encoding the artificial M1GS gene which contain different lengths of bridge sequences were amplified with PCR method.
方法以含大肠杆菌核酶P催化单位(M1 RNA)基因的pFL117质粒作为模板,通过巧妙设计不同的下游引物,经PCR扩增、扩增产物克隆及克隆基因的体外转录,构建一组具有不同桥序列的人工M1GS核酶。
3.
Objective:To explore the cleaved ability of RNase P target HCMV UL97 mRNA in vitro.
目的　探讨核酶P对HCMVUL97mRNA的体外切割能力。
2)  RNase P
RNaseP
1.
To research technique for gene silence and antiviral agents, ribozyme M1GS-T6 was constructed from external guide sequences(EGSs)that consist of a sequence complementary to HCMV UL54 gene RNA and M1 RNA, the catalytic RNA subunit of RNase P from Escherichia coli.
为研究病毒基因沉默工具和抗病毒制剂,以人巨细胞病毒UL54基因mRNA序列设计互补的外部引导序列,共价结合到大肠杆菌来源RNaseP催化核心M1RNA上,从而构建成M1GS-T6核酶。
2.
tRNase Z,RNase P and tRNA splicing endonuclease are three major endonucleases that participate in pre-tRNA processing.
tRNaseZ、RNaseP和tRNA剪接内切酶是参与tRNA前体加工的三种主要的核酸内切酶,分别参与tRNA前体3'末端、tRNA前体5'末端和内含子剪接的加工。
3)  RNase P
RNase P
1.
Targeted in vitro Cleavage of Human Cytomegalovirus UL54 mRNA Segments by RNase P;
RNase P核酶对人巨细胞病毒UL54基因mRNA体外切割作用
2.
RNase P was a ribonucleoprotein complex, which was responsible for the 5 maturation of precurosor tRNAs, and abroad existed in Archaebacteria, bacterium, yeast and animals.
RNase P是一种核蛋白复合物，广泛存在于古细菌、细菌、酵母和动物体内，它可以特异性地剪切tRNA前体(ptRNA)的5’末端，形成成熟的tRNA。
4)  RNase P
核糖核酸酶P
5)  RNase P
核酶 RNase P
6)  RNase P
核酶RNaseP
参考词条
补充资料：核酶
分子式：
CAS号：

性质：又称核酶，一类具有酶活性的核糖核酸。天然存在或人工合成的具有酶活性的小分子RNA，如锤头酶、发夹酶等。它们与相应的底物RNA结合，可行使切割功能，帮助人们了解特定基因的转录、复制，转录后加工，表达及其功能等，并可用于降解特定的mRNA，控制多种病毒感染，以及用于癌症的治疗。

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