1) PCR
![点击朗读](/dictall/images/read.gif)
测序分型
1.
High resolution DNA typing for HLA-DRB1 genes by PCR-SBT method in Han nationality in Liaoning;
辽宁地区汉族人群HLA-DRB1位点的测序分型研究
2) DNA sequencing based typing (SBT)
![点击朗读](/dictall/images/read.gif)
DNA测序分型
3) Sequence Based Typing(SBT)
![点击朗读](/dictall/images/read.gif)
测序分型(SBT)
4) Multilocus sequence typing
![点击朗读](/dictall/images/read.gif)
多位点测序分型
1.
In 1998,Multilocus sequence typing was proposed which is a nucleotide sequence based approach.
多位点测序分型(Multilocus sequence typing,MLST)技术是一种以核苷酸序列为基础的病原菌分型方法,它是高通量测序技术与成熟的群体遗传学相结合的产物。
5) sequencing based type
![点击朗读](/dictall/images/read.gif)
直接测序分型
1.
Methods A total of 88 umbilical cord blood samples,including those randomly selected from Tianjin stem cell bank and already matched for transplantation,were performed polymerase chain reaction sequence specific primers(PCR-SSP) and sequencing based type (SBT) to check their type results and sequencing based type.
方法采用聚合酶链反应-序列特异性引物扩增(PCR-SSP)方法、直接测序分型穴SBT雪方法,随机抽查天津市脐带血造血干细胞库脐带血及查询已选中做脐带血移植的样本共88例SSOP方法的结果进行HLA基因分型复核。
6) sequencing
[英]['si:kwənsiŋ] [美]['sikwənsɪŋ]
![点击朗读](/dictall/images/read.gif)
测序分析
1.
The sequencing results showed HLA-B alleles of the proband as B*4002 and the novel allele.
先证者为脐血造血干细胞捐献者,样本DNA抽提采用PEL-FREEZ抽提试剂盒,利用单链特异性引物PCR方法扩增先证者HLA-B基因的第2-4外显子,对PCR产物直接进行第2、3、4外显子双向测序分析。
2.
The sequencing results showed HLA-A alleles of the proband as A*1101 and the novel allele.
样本DNA抽提采用PEL-FREEZ抽提试剂盒,利用单链特异性引物PCR方法扩增先证者HLA-A基因的第2-4外显子,PCR产物直接进行第2、3、4外显子双向测序分析。
补充资料:序分
【序分】
(术语)诸经三分之一。(参见:序正流通三分)。
(术语)诸经三分之一。(参见:序正流通三分)。
说明:补充资料仅用于学习参考,请勿用于其它任何用途。
参考词条