Anti-inflammation,analgesic and anti-diarrhea effect of volatile oil from A.longiligulare.T.L.Wu

METHODS: Analgesic effect was investigated by hot plate test and writhing test, anti-inflammation effect by auricular swelling test in mice and toe swelling test in rat, anti-diarrhea effect by diarrhea test induced by rhubarb, effect on gastric emptying by phenolsulfonphthalein empty test in mice and effect on small intestine propulsive test by charcol powder propulsive rate test.

An orthogonal test design was applied to confirm the optimum condition of extracting the active products from Semen holarrhenae by selecting extracting temperature,extracting time and ethanol concentration,and did some research on bacteriostasis to extracts.

A HPLC method for determination of bergenin in Zhixie capsules was established.

Analysis of clinical curative effect of combination of Shuanghuanglian injection and Zhixie Decoction in the treatment of Child autumn diarrhea;

Determination of Gallic Acid in Zhixie Granules by HPLC;