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1)  pGL3-control
质粒pGL3-control
2)  PGL3 carrier
pGL3载体
1.
METHODS: The hTERT promoter and TK gene were cloned, andwhich was connected to the PGL3 carrier after enzyme digestion, toconstruct the hTERTp/PGL3、TK/PGL3、hTERTp/TK/PGL3 recombinantcarriers, and then to construct the CMV/TK/PGL3 recombinant carrierswith the similar method and regard CMV a.
方法:克隆hTERT启动子及TK基因,酶切后连接到pGL3载体上,构建hTERTp/pGL3,TK/pGL3,hTERTp/TK/pGL3重组载体。
3)  pGL3-Basic vector
pGL3-Basic载体
1.
Then P4 promoter gene fragment was inserted into the KpnI-HindⅢsite of pGL3-Basic vector,to construct pGL3-Basic eukaryotic expression vector bearing P4 promoter gene,pGL3-P4,which was further confirmed by double enzyme digestion and DNA sequencing.
成功克隆IGF-ⅡP4启动子基因,并构建P4启动子的pGL3-Basic载体。
2.
After three promoter segments with different lengths were amplified by PCR amplification,and then the products were identified by DNA sequencing,four promotor segments were inserted into pGL3-Basic vectors.
方法运用在线软件Cister对CCDC72基因5’端2000bp进行启动子特征分析;以人毛乳头细胞基因组DNA为模板进行PCR扩增目并进行测序鉴定,再以扩增产物为模板PCR得到不同长度的启动子片段,并测序,将获得启动子片段插入pGL3-Basic载体。
4)  pGL3-p21p vector
pGL3-p21p载体
5)  Hartman Loop~(TM) control
Hartman Loop~(TM)Control
6)  Command Control Communication & Intelligence
Command Control Communication&Intelligence
补充资料:control delivery
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性质:在药物缓释系统中,以特制的薄膜、胶囊或其他方式,使药物以可控制的速度释放并为人体吸收,在血液中维持较为恒定的血药浓度,取得较好的治疗效果,通过改变材料的种类、制备不同厚度的薄膜,可以有效地控制药物释放速度,理想的控制释放是零级释放。

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