1) K562/Dox cell
K562/Dox细胞
1.
OBJECTIVE To develop a method using HPLC with fluorescence detector for determining the Doxorubicin concentrations in K562/DOX cell nuclei.
方法分离K562/DOX细胞核,多柔比星浓度测定采用Diamond C18色谱柱,甲醇-水-醋酸(50∶50∶0。
2.
Methods To detect the survival rate of K562/DOX cell,which was dealt with ADM alone or with both ADM and Chinese Magnolivine Fruit Extraction.
方法MTT法检测单独应用阿霉素或阿霉素与五味子提取物联合作用的K562/DOX细胞的生存率;RT-PCR检测MDR1、MRP1 mRNA表达;Westernblotting检测MDR1蛋白的表达。
2) K562/DOX cell line
K562/DOX细胞株
3) MCF-7/DOX cell
MCF-7/DOX细胞
1.
RT-PCR assay and immunity histochemistry assay were used to determine the expression level of mdr1 gene and P-gp in MCF-7/DOX cells to elucidate resistant character of MCF-7/DOX cells.
首先以RT-PCR和免疫组化方法分别从RNA和蛋白水平检测MCF-7/DOX细胞P-gp表达情况,以明确MCF-7/DOX细胞的耐药特征;然后采用MTT法检测异汉防己碱的内在细胞毒性及其对阿霉素(DOX)的增敏作用,并以RF(reversal fold)值评价其逆转效果;同时应用流式细胞仪(FCM)对细胞内DOX的蓄积量进行了分析;再以免疫组化方法检测异汉防己碱对MCF-7/DOX细胞P-gp表达水平的影响;最后采用罗丹明蓄积和外排试验检测了异汉防己碱对P-gp功能的影响。
4) K562 cell
K562细胞
1.
An experimental study on reconstruction of retrovirus vector in human leukemia K562 cell xenograft tumor of nude Mice;
重组逆转录病毒RV-40AS对K562细胞裸鼠移植瘤的实验研究
2.
Effect of total saponins of panax ginseng on the expression of erythropoietin receptor in K562 cells;
人参总皂苷对K562细胞红细胞生成素受体表达的影响
3.
Study on apoptosis of K562 cells staining with Annexin V/PI induced by high-voltage pulse electric field;
Annexin V/PI双标记检测高电压脉冲电场诱导K562细胞凋亡的研究
5) K562 cell line
K562细胞株
1.
Cytotoxicity of cord blood monocyte-macrophage activated by cytokines against K562 cell line;
细胞因子激活的脐血单核细胞对K562细胞株杀伤作用的观察
2.
Aim To explore the effect of 4-amino-2-trifluoromethyl-phenyl retinate(ATPR)on proliferation,differentiation activity in K562 cell line,and to research the mechanisms.
目的本研究探讨新型维甲酸衍生物4-氨基-2-三氟甲基苯基维甲酸酯(4-amino-2-trifluoromethyl-phenyl retinate,ATPR)对K562细胞株的抑制增殖和诱导分化活性并对其机制进行研究。
6) K562 cells
k562细胞
1.
Differentiation of K562 cells into DC-like cells induced by the combination of calcium ionorphore with rhGM-CSF and rhIL-4;
钙离子载体促进K562细胞诱导分化成DC样细胞的研究
2.
Anti-ABL tyrosine kinase intrabody inhibits growth of K562 cells in vitro;
抗ABL酪氨酸激酶区胞内抗体抑制K562细胞体外生长
3.
Effects of the main extracts of Astragalus membranaceus on inducing the erythroid differentiation of K562 cells;
黄芪不同提取物对K562细胞向红系分化的诱导作用研究
补充资料:Czapek-Dox medium
分子式:
CAS号:
性质:又称查氏培养基。实验室常用的一种培养各种腐生真菌和土壤细菌等的合成培养基。主要成分为蔗糖、硝酸钠、磷酸氢二钾、硫酸镁、氯化钾和硫酸亚铁等,若加入凝固剂琼脂,就可以配制成固体培养基。
CAS号:
性质:又称查氏培养基。实验室常用的一种培养各种腐生真菌和土壤细菌等的合成培养基。主要成分为蔗糖、硝酸钠、磷酸氢二钾、硫酸镁、氯化钾和硫酸亚铁等,若加入凝固剂琼脂,就可以配制成固体培养基。
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