1) cDNA microarray hybridization
表达谱基因芯片杂交
2) cDNA microarray
基因表达谱芯片
1.
Subsequently, the cDNA probes were hybridized to the Mouse40S cDNA microarray and the fluorescent signals .
方法 NP染毒F0亲代母鼠 ,分别提取NP组和对照组F1子代雄性 2日龄大鼠的脑组织mR NA ,分别用cy5和cy3逆转录标记 ,与Mouse4 0S基因表达谱芯片杂交 ,并对cy3、cy5荧光信号做扫描分析。
2.
Objective:To decipher the antitumor mechanisms of Chinese compound Jinlongshe(JLS) granule using cDNA microarray to identify gene expression changes in MKN-45 human gastric cancer.
目的:利用基因表达谱芯片研究金龙蛇颗粒对MKN-45胃癌的分子机制。
3.
cDNA microarray is now regarded as being effective for exploring the mechanism of the effect of medicine.
针对D-半乳糖造成的小鼠亚急性衰老模型,采用Cy3和Cy5 2种不同的荧光染料,通过逆转录反应将实验组和对照组的小鼠脑组织细胞mRNA分别标记成2种探针,混合后与小鼠基因表达谱芯片杂交,借助计算机分析扫描芯片荧光信号图像,寻找出经人参作用后表达有显著差异的基因,并对这些基因进行了生物信息学分析。
3) Microarray expression profiling
基因芯片表达谱
4) gene chip
基因表达谱芯片
1.
Methods Searching for the alterably expressed genes before or after 10 mg/L sulfide by gene chip in NB4 and testing the results by RT-PCR.
方法应用基因表达谱芯片检测NB4细胞在10mg/L硫化砷作用前后的基因表达改变,RT-PCR方法验证基因表达谱芯片结果和检测白血病原代细胞中PNAS2基因的表达情况。
2.
Methods Gene chip was employed to detect the genes related to AngRem104 by its over-expressed constructs, which was produced by transfection of the sense- and antisense-AngReml04 into human mesangial cells, and then RT-PCR was used to confirm the up-regulated expression of related genes.
结论 应用作为研究基因功能有效手段的基因表达谱芯片技术来筛选新基因AngRem104的功能相关基因,发现AngRem104与FN的表达有关,为其功能研究提供了重要线索。
5) gene expression profile
基因表达谱芯片
1.
The cDNA obtained by reverse transcription polymerase chain reaction (RT-PCR),was labeled with Cy5 and Cy3 fluorescence as probes,and then hybridized with gene chip containing 21522 human 22K gene expression profile.
纯化mRNA,逆转录合成cDNA标记后,与22K人类基因表达谱芯片进行杂交,扫描后筛选出表达差异的基因。
2.
The cDNA was obtained by reverse transcription polymerase chain reaction (RT-PCR),and then labeled with fluorescence as probes,which were hybridized with gene chip containing human 22K gene expression profile.
目的利用基因表达谱芯片技术筛选过表达E2F-1的胃癌MGC-803细胞与对照组间差异表达的免疫相关基因,初步探讨E2F-1对胃癌细胞免疫功能影响的机制。
6) expressional profiles microarray
表达谱基因芯片
1.
Objective Identification of the differently expressed genes on human leukemic K562 cells induced with sodium butyrate(NaB)by expressional profiles microarray,screening an associated novel gene related with erythroid lineage and investigation the pharmaco-logy effects of NaB.
结果表达谱基因芯片结果分析显示:总探针组54675个芯片中,阳性表达数23175(42。
补充资料：基因表达系列分析
基因表达系列分析（sage）是通过快速和详细分析成千上万个est（express sequenced tags）来寻找出表达丰富度不同的sage标签序列。再次访法中，通过限制性酶切可以产生非常短的cdna（10-14bp）标签，并通过pcr扩增和连接，随后对连接题进行测序。sage大大简化和加快了3’端表达序列标签的收集和测序。同dd一样，sage是一个“开放”的系统，可以发现新的未知的序列。在进行标本的比较之前，sage在cdna的产生和处理上需要较多个步骤。由于sage是一个依赖dna测序的基因计量方法，它对基因表达的测定比dd更加量化。由于需要进行大量的测序反应，所以费用因素使大多数研究机构对其广泛应用的主要限制。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条