1) fragment length discrepant allele specific PCR
片段长度差异等位基因特异性PCR
1.
Objective To establish a new method for single nucleotide polymorphism (SNP) typing based on allele specific PCR: fragment length discrepant allele specific PCR (FLDAS-PCR), and study the influence on specific extension by introducing a mismatch at the third or fourth 3′-terminal base of allele specific primers.
目的建立一种基于等位基因特异性PCR原理的改良SNP分型新方法:片段长度差异等位基因特异性PCR,并考察特异性引物的3'端第3位、第4位碱基错配对特异性延伸的影响。
2) Fragment length discrepant allele specific PCR (FLDAS-PCR)
片段长度差异等位基因特异性PCR(FLDAS-PCR)
3) allele different specific PCR
等位基因差异特异性PCR
1.
Genotyping of hepatitis B virus DNA precore 1 896 site mutation by allele different specific PCR and its clinical significance;
等位基因差异特异性PCR检测HBVDNA的前C区1896突变位点及其临床意义
4) allele-specific PCR
等位基因特异PCR
1.
Detection of mtDNA A1555G mutation by allele-specific PCR and its melting curve analysis
等位基因特异PCR结合熔解曲线分析快速检测线粒体DNA A1555G突变
5) Allele specific-PCR
等位基因特异性-PCR
1.
Detection of JAK2V617F Mutation by Allele specific-PCR and Restriction Enzyme Digestion;
等位基因特异性-PCR联合限制性内切酶消化法检测JAK2V617F突变
6) allele-specific PCR
等位基因特异性PCR
1.
Development of allele-specific PCR assay for detecting Salmonella pullorum;
鸡白痢沙门菌等位基因特异性PCR检测方法的建立
2.
An allele-specific PCR-based method was developed to distinguish Fusarium virguliforme,the causal agent of soybean sudden death syndrome(SDS) in North America,from Fusarium phaseoli.
应用等位基因特异性PCR(allele-specific PCR,AS-PCR)技术,建立了大豆猝死综合症(sudden death syndrome of soy-bean,SDS)病原菌北美种Fusarium virguliforme与其近似种Fusarium phaseoli(菜豆根腐病菌)的鉴别方法。
补充资料:限制性片段长度多态性
分子式:
CAS号:
性质:又称限制片长多态性。每一种基因型的DNA对某些限制性内切酶有固定的酶切位点,经酶作用后,在凝胶电泳图谱上产生固定的片段图谱。如基因型发生变化,则酶切位点或数目可能有差异,则产生的电泳图谱会有差异。这方法常用于遗传基因型的检查或病理检查。一般来讲高等生物个体都有特定的RFLP。
CAS号:
性质:又称限制片长多态性。每一种基因型的DNA对某些限制性内切酶有固定的酶切位点,经酶作用后,在凝胶电泳图谱上产生固定的片段图谱。如基因型发生变化,则酶切位点或数目可能有差异,则产生的电泳图谱会有差异。这方法常用于遗传基因型的检查或病理检查。一般来讲高等生物个体都有特定的RFLP。
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参考词条