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1)  DNA pool
DNA池
1.
Four breeds of chickens (Gallus gallus), White Leghorn, Yangshan, Taihe Silkies and White Recessive Rocks ,with different egg production were applied to screen potential haplotype related to reproduction trait in part region of prolactin gene based on DNA pooling and sequencing.
选取产蛋性能具有明显差异的4个鸡(Gallus gallus)品种(莱航鸡、阳山鸡、丝羽乌骨鸡和隐性白洛克鸡)构建品种DNA池,通过测序研究鸡催乳素基因5'-侧翼调控区部分序列(1028bp)的单倍型,快速筛查到由4个单核苷酸多态性(SNP)位点(C-2402T、C-2161G、C-2062G和G-2040A)组成的2种最常见单倍型CCCG和TGGA,其中CCCG在莱航鸡品种中的频率接近1,可能利于产蛋。
2.
DNA was extracted from these seven breeds and DNA pools were constructed respectively.
以7个警犬品种DNA池为试验材料,首次建立了稳定的犬基因组扩增片断长度多态性(AFLP)指纹图谱检测方法。
3.
Forty-eight DNA pools were prepared using standardized DNA extracted from 152 Ganoderma isolates (128 Chinese isolates and 24 non-Chinese isolates) delineated on the basis of ERIC-PCR data.
根据ERIC聚类分析的结果,把152株灵芝属菌株(包括128株来自中国的栽培菌株及24株国外菌株)建成48个DNA池
2)  DNA pooling
DNA池
1.
To investigate the relationship between the single nucleotide polymorphism (SNPs) of the bcr and abl gene and chronic myelogeous leukemia (CML), the 9 sequence-tagged sites (STS) in bcr and abl gene were screened by DNA pooling and denaturing high performance liquid chromatography (dHPLC), and the results were varified by sequencing.
为了探讨bcr和abl基因的单核苷酸多态性(SNP)与慢性髓细胞性白血病(CML)的关系,利用DNA池(DNApooling)结合变性高效液相色谱(dHPLC)技术对bcr和abl基因上的9个序列标签位点(sequencetaggedsite,STS)进行序列变异的筛查分析,并通过测序对筛查结果进行验证。
2.
Four breeds of chickens (White Leghorn,Yangshan,Taihe Silkies,White Recessive Rocks) with different egg production were applied to screen potential single nucleotide polymorphisms (SNPs) related to reproduction trait in distal part region of prolactin gene based on DNA pooling and sequencing.
选取产蛋性能具有明显差异的 4个鸡品种(莱航鸡、阳山鸡、丝羽乌骨鸡和隐性白洛克鸡 )构建品种DNA池,采用测序的方法研究鸡催乳素基因 5′侧翼调控区远端序列 (1 028bp)的多态性,快速筛查到 8个可能与产蛋性能相关的SNPs(C 2402T、T 2192C、C 2161G、C 2134G、C 2062G、G 2040A、A 1944G和C 1884A)。
3)  pooled DNA
池DNA
1.
Based on the similarity index of pooled DNA.
根据池DNA所获得的品种间相似指数计算遗传距离。
4)  DNA pooling
DNA混合池
1.
Methods A total of 30 microsatellite markers on chromosome 2 spaced at about 10 cM were selected and two separated DNA pooling samples consisting of 450 essential hypertension cases and 450 normal controls were genotyped respectively.
方法用DNA混合池(DNApooling)方法,在2号染色体上间隔10cM(厘摩)遗传距离选择30个微卫星遗传标记,对450例EH患者和450例正常对照者组成的DNA混合样本分别进行扫描。
5)  Polled DNA
混合DNA池
6)  calf thymus DNA
DNA
1.
The interaction of three chosen non-steroidal anti-inflammatory drugs (NSAID), indomethacin, sulindac and tolmetin with calf thymus DNA has been investigated by fluorescence with the use of fluores- cence probes, ethidium bromide (EB) and neutral red (NR).
采用溴化乙锭(EB)和中性红(NR)两种荧光探针分别考察了三种非甾体抗炎药(吲哚美辛、舒林酸和托美丁)与DNA的相互作用,采用荧光发射光谱和共振散射光谱技术得出了基本一致的结论:吲哚美辛和舒林酸可以在一定程度上与DNA结合(舒林酸更强),结合方式可能有嵌插作用,也可能有药物分子在DNA双链表面的组装,作用力来源于静电作用。
2.
The interaction of the complex with calf thymus DNA have been investigated by electronic and emission spectra, and the results shown the complex bind to DNA with high affinity.
目的与方法合成了一种新型钌多吡啶配合物 [Ru(phen) 2 CPIP](PF6) 2 ,采用元素分析、核磁共振和电喷雾质谱对配合物进行表征 ,并采用电子吸收光谱和荧光光谱对配合物与DNA相互作用的性质进行研究。
补充资料:DNA分子花窗(沿B―DNA轴线方向看)




DNA分子花窗(沿B―DNA轴线方向看)
  [图]图

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