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1)  luciferase reporter gene
荧光素酶报道基因
1.
Objective To determine the possibility of telomere seeding in human gastric cancer cell SGC7901 and the influence of telomere on the expression of luciferase reporter gene nearby and to explore the position effect of telomere in gastric cancer cell.
目的 研究插入端粒片段的荧光素酶报道质粒在胃癌细胞的转染及它对相邻荧光素酶报道基因表达的影响,探讨胃癌细胞中端粒位置效应的存在。
2)  luciferase reporter gene
荧光素酶报告基因
1.
These DNA fragments were inserted into the pGL3-Basic which was the vector of luciferase reporter gene.
方法提取人内皮细胞基因组DNA,设计引物克隆EOLA1基因上游不同长度的基因片段,插入到含荧光素酶报告基因的载体pGL3-Basic中,经限制性内切酶酶切鉴定、测序。
2.
[Method] Clonging the promoters of CYP3A4 and CYP2B6 which contained the elements that hPXR, a kind of nuclear receptor, can recognize and bind, and inserting the trans-element to the upstream of firefly luciferase reporter gene of the pGL4.
方法利用双荧光素酶报告基因系统,将包含hPXR蛋白识别和结合调控元件的CYP3A4和2B6启动子序列插入报告基因上游,将表达载体和报告载体共转染HepG2细胞,用含有利福平或DMSO培养基培养48h后裂解进行双荧光素酶活性检测。
3.
Amplified apolipoprotein A-I(apoA-I) promoter gene(376 bp) containing essential transcription regulatory elements from human genomic DNA was fused upstream to the luciferase reporter gene and neomycin resistance gene in the constructed pGL3B-neo vector.
PCR扩增含有apoA-I基因的转录调控序列的启动子片断(376 bp),克隆入含有荧光素酶报告基因和新霉素抗性基因的pGL3B-neo载体中,构成受apoA-I启动子调控的重组报告基因质粒pGL3B-neo::apo,稳定转染人肝癌细胞株HepG2。
3)  luciferase report gene
荧光素酶报告基因
1.
Methods The +495bp~+584bp of SCN5A promoter was amplified by polymerase chain reaction(PCR)and recombinated into pGL3-promoter luciferase report gene vector and transient transfected HEK293 cell and H9C2 cell.
方法应用聚合酶链反应扩增大鼠心肌SCN5A基因启动区+495bp~+584bp片段,与荧光素酶报告基因载体pGL3-promoter重组,以pGL3-promoter空载体为对照,瞬时转染人胚肾母细胞(HEK293)和小鼠胚胎心肌细胞(H9C2),检测荧光素酶活性。
2.
coli chromosome,the luciferase report gene was knocked in lacZ site of chromosome lac operon by using Red recombination system and selection-counterselection kan/sacB technology.
为了应用Red重组工程技术实现外源基因在大肠杆菌染色体上的表述,寻找染色体上外源蛋白的稳定高效表达位点,使用Red重组工程系统和kan/sacB无痕迹修饰技术,将易于定量分析的荧光素酶报告基因替换DY330染色体lac操纵子中的lacZ基因。
4)  dual-luciferase report gene
双荧光素酶报告基因
5)  Jun-2 luciferase report gene
Jun-2荧光素酶报告基因
6)  luciferase reporter gene plasmid
荧光素酶报告基因质粒
1.
AIM:To construct and identify luciferase reporter gene plasmid containing hBD-2 and its mutant correlated with Crohn\'s disease.
目的:构建人β-防御素-2(hBD-2)荧光素酶报告基因质粒及其克罗恩病(Crohns,CD)相关突变体,并检测其在人胚胎肾T细胞(HEK-293T)中的活化。
补充资料:可溶性荧光素 ,荧光素钠
药物名称:荧光素钠

英文名:Fluorescein Sodium

别名: 可溶性荧光素 ,荧光素钠
外文名:Fluorescein Sodium
适应症: 1.滴眼液用于眼科诊断,正常角膜不显色,异常角膜显色。 2.针剂用于测血液循环时间,静注后,在紫外线灯下观察,以10~16秒内唇部粘膜能见到黄绿色荧光为正常
用量用法:
1.滴眼后于角膜显微镜下观察颜色。 2.测血循环时间,于臂静脉注2ml,每次用量0.4~0.8g(2~4ml)
注意事项: 滴眼液应注意灭菌并防止污染
规格: 滴眼液:2% 注射液:0.4g(2ml)


类别:实验诊断用药
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