Experimental study on apoptosis of ovarian granulosa cells of radiated mice treated by Chinese angelia injection;

Culture of mouse granulosa cells in vitro;

The apoptosis rates of granulosa cells were detected by the TdT-mediated dUTP-biotin nick end Labeling(TUNEL) and preparation of paraffin section on days 1 and 3 post-injection respectively,in order to investigate the change of lead on the ovarian structure and apoptosis of granulose cells mice.

The effct of TNF-α on apoptosis of ovary granulosa cells of rats with hysterectomy;

The development of nuclear transfer(NT)embryos derived from adult ear fibroblasts, fetal fibroblasts, fetal skin cells and ovary granulosa cells were compared.

Objective To investigate the effects of Er Xian Decoction(EXD)and its three subdivisions“wanning Shen”,“nourshing Yin”and“adjusting Chong and Ren”in regulating the level of estradiol(E_2)on the primary cultured granulosa cells.

To elucidate the effects of pregnant mare serum gonadotrophin(PMSG) on follicle development,atresia and apoptosis of granulosa cells,changes of follicle number on the ovarian surface,proportions of apoptotic granulosa cells in follicles of different size and changes in serum estrogen and progesterone level at 24 hr,48 hr,72 hr and 96 hr after PMSG injection in immature gilts were studied.