1) immunofluorescent double staining
免疫荧光双染
2) Double immunofluorescence staining
免疫荧光双重染色
1.
Double immunofluorescence staining with monoclonal antibodies(1:100) against phospho-histone H3 and α-sarcomeric actin was performed on the cultured cells.
方法对H9c2(2-1)大鼠心肌细胞进行培养,利用免疫荧光双重染色技术,用横纹肌肌动蛋白IgM单克隆抗体标记心肌细胞,用磷酸化组蛋白H3 IgG单克隆抗体标记有丝分裂的染色体,同时用Hochest 33342显示细胞核,荧光显微镜观察并摄片。
3) Double color immunofluorescence
双色免疫荧光染色
4) immunofluorescence double staining technique
免疫荧光双染技术
5) double immunofluorescence
双重免疫荧光染色
1.
Sarcolemma-specific collagen Ⅵ deficiency can be better demonstrated by double immunofluorescence staining.
方法收集2例Ⅵ型胶原肌膜选择性缺失型UCMD患者的临床资料,进行肌肉活体组织检查,标本行Ⅵ型胶原免疫荧光染色和Ⅵ型胶原/Ⅳ型胶原双重免疫荧光染色,对病理结果进行分析。
6) immunofluorescence staining
免疫荧光染色
1.
Methods Cells were harvested at 0 day,the 3rd day and the 7th day after BGSCs differentiation,then the ratio of Hes-1 positive cells were counted by flow cytometry,and the expression intensity and cell types were detected by laser confocal microscopy after immunofluorescence staining with Hes-1 and GFAP,MAP2 or MBP.
方法对体外培养的人脑胶质瘤干细胞进行诱导分化,于未分化、分化第3、7d收集标本,行Hes-1蛋白免疫荧光染色后流式细胞术检测标本中阳性细胞比例;Hes-1分别与CD133、GFAP、MAP2、MBP蛋白双标免疫荧光染色后,激光共聚焦显微镜下观察Hes-1蛋白表达强度及细胞类型的变化。
2.
The nNOS neurons were detected by immunofluorescence staining.
用免疫荧光染色、RT-PCR和Western blot方法对nNOS神经元数量、nNOS基因和蛋白的表达量进行检测。
3.
The aim of this experiment was to study the effective method of fixation and penetration before immunofluorescence staining in oocytes and early embryos to raise the effect of immunofluorescence staining in them.
本实验旨在探索有效的染色前固定和渗透卵母细胞及早期胚胎的方法,以提高小鼠卵母细胞和早期胚胎免疫荧光染色的效果;同时检测RNA聚合酶Ⅱ(PolⅡ)在小鼠卵母细胞和早期胚胎的表达,探讨PolⅡ在卵母细胞和早期胚胎发育中的作用。
补充资料：精子间接免疫荧光法
精子间接免疫荧光法
诊法。免疫学检查方法之 一。用于不育症检查。①以志愿供者的精液02ml加入10～12ml生理盐水中离心，取其精子沉淀部分再置入05ml生理盐水内。②取一滴作玻璃涂片，并在空气内晾干30分钟，用纯甲醇固定30分钟，再晾干后置入pH为74的磷酸缓冲液内15分钟。③被测血清滴于上述制备的玻片上，室温下孵化30～60分钟后，用磷酸缓冲液漂洗3次，每次10分钟。④玻片上滴注 附有抗人体免疫球蛋白抗血清的异硫氰酸荧光素盐酸盐，室温下孵化30～60分钟，孵化后用磷酸缓冲液漂洗3次，每次10分钟。⑤荧光显微镜下观察结果。利用血清内的精子抗体，可使精子不同部位呈现不同免疫荧光素反应，通过与正常血清对比研究，以判断精子抗体的存在情况。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条