Methods The gene encoding HSP65 and G2 glycoprotein was amplified from genome of mycobacterium tuberculosis and T-H8205G2 plasmid by polymerase chain reaction(PCR).

The G2 glycoprotein gene was amplified and cloned into the plasmid vector pCDNA3 1/His containing CMV promoter by using RT PCR and molecular cloning technique,the recombinant plasmid G2 pCDNA3.

AIM: To express hantaan virus(HTNV) envelope glycoprotein G_2 recombinant adenovirus(Adeno-G_2)in vero E6 cells and explore its property of inducing immune response.

Study on the interaction of Hantavirus envelope G2 protein with β3 intergrin in vivo;