1) TOPO-TA cloning
TOPO-TA克隆
1.
Methods PCR,TOPO-TA cloning ,molecular hybridization techniques and corresponding detecting system were used to evaluate the differential gene expression profiles of GlcNAc6ST,GalT and GnT in different tumor cell lines.
方法TOPO-TA克隆获得硫酸基(磺基)转移酶、半乳糖基转移酶和N-乙酰氨基葡萄糖转移酶三种糖基转移酶基因家族的cDNA文库,PCR获得目的基因,逆转录标记不同肿瘤细胞株的cDNA探针,化学发光法表达阵列对人不同肿瘤细胞进行表达谱分析。
2.
Methods Mainly by means of PCR, TOPO-TA cloning and molecular hybridization techniques.
方法主要为PCR，TOPO-TA克隆和分子杂交技术。
2) TOPO cloning
TOPO克隆
1.
In this expriment, the GFP gene was amplified by PCR from the plasmid pEGFP-N1, then it was ligated into the pLenti6/V5-D-TOPO by the TOPO cloning efficiently, quickly and directly, and constructed the recombined pLEGFP vector that could transfect the cells efficently and display protein expressing expediently.
实验利用PCR(polymerase chain reaction)从质粒pEGFP-N1中扩增了EGFP基因片段,再利用高效、快速、定向的TOPO克隆法将扩增片段克隆到pLenti6/V5-D-TOPO载体中,构建了既能高效转染细胞又能方便观察蛋白表达情况的pLEGFP重组载体。
2.
Methods: TOPO cloning technique was selected to construct the lentivirus vector: pLenti6/V5-EGFP-MxA.
方法:本实验利用TOPO克隆技术,构建EGFP-MxA融合基因的重组慢病毒表达载体,经PCR及酶切鉴定EGFP-MxA插入载体的方向正确,且没有引起基因重排;重组载体导入真核细胞293FT细胞后,能表达EGFP-MxA融合蛋白特征性的颗粒状绿色荧光。
3) TA clone
TA克隆
1.
After TA clone,vector construction of Cui-SS,Cui-155 baculovirus and transposition,recombinant baculovirus were obtained.
方法 :应用 RT- PCR法对全 NP蛋白基因及其型特异区基因进行扩增 ,经 TA克隆及 Cui- SS、Cui- 15 5杆状病毒载体的构件、转获重组杆状病毒 ,再感染 Sf9细胞进行表达。
2.
After TA clone,vector construction fo Cui-ss,Cui-155 baculovirus and transposition,recombinant baculovirus was obtained .
方法 应用RT -PCR法对全NP蛋白基因及其型特异区基因进行扩增 ,经TA克隆及Cui-SS、Cui- 15 5杆状病毒载体的构件、转座、获重组杆状病毒 ,再感染Sf9细胞进行表达。
5) TA-cloning
TA法克隆
6) TA cloning vector
TA克隆载体
1.
Construction of the recombinant pET15b-SOD1 plasmid with TA cloning vector;
利用TA克隆载体构建pET15b-SOD1重组质粒
2.
Construction of rat antisense TIMP-1 gene TA cloning vector;
大鼠金属蛋白酶组织抑制物-1反义基因片段TA克隆载体的构建
3.
The PCR product was cloned into TA cloning vector.
结论 成功地构建了 HLA-DRα基因片段TA克隆载体。
补充资料：TOPO
分子式：C24H51OP
分子量：386.64006
CAS号：78-50-2
性质：暂无
制备方法：暂无
用途：暂无
分子量：386.64006
CAS号：78-50-2
性质：暂无
制备方法：暂无
用途：暂无
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条