1) multiplex reverse transcription-PCR
多重逆转录聚合酶链反应
1.
Method:Multiplex reverse transcription-PCR and multiplex-PCR were used to amplify and differentiate the hemagglutinin (HA) genes of H 1,H 3 and B influenza virus.
方法 :用多重逆转录聚合酶链反应连接多重聚合酶链反应扩增甲 1 、甲 3和乙型流感病毒的 HA基因的 HA1 片段。
2) MRT-PCR
多重逆转录聚合酶链反应
1.
Objective A multiplex reverse transcription polymerase chain reaction (mRT-PCR) method is capable of detecting and subtyping the main respiratory viruses,including influenza A (H1N1 and H3N2) and B viruses as well as respiratory syncytial virus (RSV) types A and B.
目的 建立用多重逆转录聚合酶链反应 (mRT -PCR)检测甲 1型、甲 3型、乙型流感病毒以及呼吸道合胞病毒 (RSV)A型、B型等主要上呼吸道病毒的方法 ,以快速检测上呼吸道感染主要病毒及对甲型、甲亚型、乙型流感病毒在上海地区人群感染、流行情况调查。
3) reverse transcription polymerase chain reaction
逆转录多聚合酶链反应
4) RT-PCR
逆转录-多聚合酶链反应
1.
E-Cad mRNA was evaluated in the above mentioned samples by semi-quantitative RT-PCR,and the correlation between E.
方法采用免疫组织化学染色的方法观察比较E-Cad在进展期胃癌原发灶及转移淋巴结中的表达;同时用逆转录-多聚合酶链反应(RT-PCR)方法检测上述标本中E-Cad基因的表达,并对其和临床病理参数的关系进行分析。
2.
Methods: Reverse tanscriptase-polymerase chain reaction (RT-PCR) method was applied to detect the expression of HMGA1-mRNA and HMGA2-mRNA and Western blot analysis respectively was applied to detect the expression of HMGA1 and HMGA2 protein in endometrial carcinoma cell lines AN3CA, ECC-1, Ishikawa and tissue specimens from cacinomatous endometrium (n=21) and normal endometrium (n=18).
方法:采用逆转录-多聚合酶链反应、免疫印迹法检测HMGA1与HMGA2在体外培养的子宫内膜癌细胞系AN3CA、ECC-1、Ishikawa以及21例子宫内膜癌组织标本(研究组)和18例正常子宫内膜组织标本(对照组)中的表达。
3.
Methods:The expressions of HMGA2-mRNA and HMGA2 protein were detected in endometrial carcinoma cell lines AN3CA,ECC-1,Ishikawa and tissue specimen from cacinomatous endometrium(n=21)and normal endometrium(n=18)using reverse tanscriptase-polymerase chain reaction(RT-PCR)and Western blot analysis,respectively.
方法:采用逆转录-多聚合酶链反应(RT-PCR)、免疫印迹法(Western Blot)检测HMGA2在体外培养的子宫内膜癌细胞系AN3CA、ECC-1、Ishikawa以及21例子宫内膜癌组织标本和18例正常子宫内膜组织标本中的表达。
5) multiplex reverse transcription-polymerase chain reaction
多重套式逆转录聚合酶链反应
补充资料：聚合酶链反应
聚合酶链反应
polymerase chain reaction,PCR
是用特异性寡核苷酸引物在DNA聚合酶的作用下对靶DNA序列进行大量扩增的分子生物学方法。PCR技术中以在短时间内把极其微量的特定DNA(或RNA）片断大量增扩，达到用常规方法就可以检测的水平。临床用来确定一些疾病的病原体，因其假阳性率较高，故应谨慎使用。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条