1) Chromatin immunoprecipitation
染色质免疫沉淀
1.
AIM: To construct a new method named PolII-ChIP-PE- MS (RNA polymerase II-Chromatin immunoprecipitation- primer extension-mass spectrum), and to validate the reliability of this method in the study of single nucleotide polymorphism (SNP) allele-specific quantification o RNA polymerase loading.
目的:建立RNA多聚酶Ⅱ-染色质免疫沉淀-引物延伸-飞行时间质谱技术(PolⅡ-ChIP-PE-MS),并利用该方法检测SNP等位特异磷酸化PolⅡ结合量,为活体状态下研究疾病关联基因SNP的功能研究提供一种高通量的研究手段。
2.
After the cells were cross-linked by formaldehyde and chromatin was sonicated by ultrasonic, we performed RNA polymerase Ⅱ-chromatin immunoprecipitations (PolⅡ-ChIP) to precipitate the promoter sequences and validated them by PCR.
方法常规培养和刺激HepG2和L02细胞,甲醛固定细胞,超声剪切染色质,采用利用PolⅡ CTD末端Ser5特异性抗体进行染色质免疫沉淀(PolⅡ-ChIP),针对文献报道的8个可能启动子区设计引物进行PCR扩增,鉴定ESR1基因启动子的使用情况。
3.
The binding of Ezh2 and H3K27me3 on the Ngn 1 promoter was analyzed using chromatin immunoprecipitation assay.
方法通过Western印迹检测全反式维甲酸(RA)诱导TuJ1蛋白的表达;通过染色质免疫沉淀技术检测Ngn1基因启动子区Ezh2的结合与H3K27三甲基化的变化;通过实时定量RT-PCR检测RA诱导P19细胞分化后Ezh2mRNA表达。
2) chromatin immunoprecipitation assay
染色质免疫沉淀技术
1.
Objective To establish a Chromatin immunoprecipitation assay to analyze transcriptional regulation of gene expression in hepatocytes.
目的建立一种染色质免疫沉淀技术(ChIP)分析肝细胞基因表达的转录调控方法。
2.
Now, chromatin immunoprecipitation assay (ChIP) is ideally suited for investigating DNA-protein interactions in vivo.
染色质免疫沉淀技术(chromatin immunoprecipitation assay,简称ChIP)是目前唯一研究体内DNA与蛋白质相互作用的方法。
3.
Nowadays, in comparison with other methods, chromatin immunoprecipitation assay (ChIP) is ideally suited for studying DNA-protein interactions in vivo.
与其他方法相比,染色质免疫沉淀技术(chromatin immunoprecipitation assay,ChIP)是一种近来研究体内DNA与蛋白质相互作用的最好方法之一。
3) chromatin immunoprecipitation assay (ChIP)
染色质免疫沉淀技术(ChIP)
4) chromatin immunoprecipitaion (ChIP)
染色质免疫沉淀(ChIP)
5) Chromatin immunoprecipitation
染色质免疫共沉淀
1.
Here we adopted Chromatin immunoprecipitation linked to microarrays(ChIP-chip),for the first time,to profile the variations in H3K9me3 in CpG island regions in squamous carcinomas of the cervix patients and controls\' ChIP-qPCR is used to validate the microarray results.
方法:收集宫颈鳞癌组织5例及对照组织5例,采用染色质免疫共沉淀联合芯片技术(ChiP-chip)在全基因组范围内对宫颈鳞癌组织及正常宫颈组织中的组蛋白H3K9me3进行高通量的筛选,获得宫颈鳞癌组织中H3K9me3的表达图谱。
6) chromatin immunoprecipitation(ChIP) assay
染色质免疫沉淀实验
补充资料：放射免疫沉淀反应
放射免疫沉淀反应
是用放射性同位素标记的抗体或抗原进行免疫沉淀反应的一种方法。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条