1) G-PAGE
复性电泳(G-PAGE)
2) refolding
复性
1.
Cooperation of osmolytes and artificial chaperone on malate dehydrogenase refolding;
渗透剂与人工伴侣耦合辅助苹果酸脱氢酶包含体的复性
2.
Effect of betaine on unfolding thermodynamics and refolding kinetics of lysozyme;
甜菜碱对溶菌酶去折叠热力学和复性动力学的影响
3.
Refolding of denatured carbonic anhydrase assisted by amide and refolding kinetics;
酰胺类物质辅助变性碳酸脱水酶复性及动力学
3) renaturation
复性
1.
Study on chromatographic renaturation of Erythrina trypsin inhibitor;
刺桐胰蛋白酶抑制剂的色谱复性研究
2.
Purification,Renaturation and Biological Activity of Recombinant Human Interleukin-18;
重组人白细胞介素-18的纯化、复性及其活性检测
3.
Purification and renaturation for the production of the encoding gene of OXA-1 subtype of extended spectrum β-lactamases by pseudomonas aerugenosa;
铜绿假单胞菌超广谱β-内酰胺酶编码基因OXA-1表达产物的纯化及复性分析
4) refold
复性
1.
Purification and Refolding of Infectious Bursal Disease Virus(IBDV) Structural Protein VP2 Expressed in Escherichia coli;
鸡传染性法式囊病毒VP2原核表达蛋白的纯化和复性
2.
Purification, refold and activity assay of FMDV structural proteinVP2-3-1 expressed in Escherichia coli;
口蹄疫病毒结构蛋白VP2-3-1的纯化复性与活性检测
3.
Using the three state model, the refolding conditions of recombiant proteins had been selected to get good results.
论述了使用蛋白质复性过程中的三态模型来选择基因重组蛋白质的复性条件,并对以往使用的复性条件和新的复性方法进行了合理的解释,为从理论上选择蛋白质的复性条件提供了依据。
5) Renature
复性
1.
PurposeTo renature the recombinant human superoxide dismutase expressed(rh SOD) in E.
结果Cu2 + 、Zn2 + 的加入对于恢复SOD的活性和稳定性是必需的 ,适当提高温度将大大缩短复性时间 ,获得的rhSOD比活可达到 6 0 0 0u/mg以上。
2.
3 proteins were denatured under three kinds of strong denaturing conditions by heat treatment (at 100℃, 15 min) or chemical reagents (12 mol/L urea or 8 mol/L guaridine, 4 hours) and then were renatured by cooling or dialysis.
用三种不同的强变性条件 (10 0℃加热15min ,12mol/L脲或 8mol/L盐酸胍处理 4h)将Hsp16 3变性 ,然后通过冷却或透析使之复性 ,并利用孔径梯度聚丙烯酰胺凝胶电泳和圆二色性光谱比较了变性 复性前后Hsp16 3的各个层次高级结构 。
6) Repeatability
重复性
1.
Analyzing Methods of Repeatability and Reproducibility in Measurement System;
测量系统重复性误差和重现性误差的分析方法
2.
Analysis on the repeatability and reproducibility of the results of density determinations of abrasive grains;
磨料密度测定结果的重复性和复现性分析
3.
A study on the repeatability of EMG of the mastication muscles through surface eletrodes and its reference values;
咀嚼肌表面电极肌电图重复性检验和参考值的建立
参考词条
补充资料:Sds-聚丙烯酰胺凝胶电泳凝胶电泳
sds-聚丙烯酰胺凝胶电泳(sds-page,sodium dodecyl sulfate-polyacrylamide gel electrohoresis)
在有去污剂十二烷基硫酸钠存在下的聚丙烯酰胺凝胶电泳。sds-page只是按照分子大小分离的,而不是根据分子所带的电荷和大小分离的。
说明:补充资料仅用于学习参考,请勿用于其它任何用途。