1) replication and expression of pIRES2-EGFP-DAZ3 plasmid
pIRES2-EGFP-DAZ3质粒复制与表达
2) pIRES2-EGFP
pIRES2-EGFP质粒
1.
Construction of μ-pIRES2-EGFP and its Expression in HEK293
大鼠μ型阿片受体的pIRES2-EGFP质粒构建及其在HEK293细胞中的表达
3) pIRES2-EGFP-4-1BBL plasmid
pIRES2-EGFP-4-1BBL质粒
1.
Result Production of the reconstructed SL3261 strain containing pIRES2-EGFP-4-1BBL plasmid was much slower than that in its predecessor,with an appereance of negative Gram staining and trichobacteria.
结果含pIRES2-EGFP-4-1BBL质粒SL3261在体外生长繁殖较原细菌慢,革兰染色呈阴性丝状菌体,含pIRES2-EGFP-4-1BBL质粒的SL3261 A-F多价、O4菌体和H1鞭毛抗原和SL3261完全一致;体外感染HepG2能力,SL3261为201±46 CFU/200HepG2细胞,SL3261-pIRES2-EGFP为163±37,而SL3261-pIRES2-EGFP-4-1BBL为158±32,3组间的差异无统计意义(P>0。
4) bicistronic pIRES-EGFP/DsRed
双顺反子表达质粒pIRES-EGFP/DsRed
5) replication and expression
复制与表达
1.
To explore possibility of hepatitis B virus(HBV)vertically transmitting via spermatozoon,to study the replication and expression of sperm-mediated HBV genes in two-cell embryos of golden hamster.
方法:将金黄地鼠精子与pBR322-HBVDNA重组质粒进行共培养后,与金黄地鼠正常卵母细胞受精,取2-细胞胚分别制片和提取RNA,用荧光原位杂交(Fluorescenceinsituhybridization,FISH)、RT-PCR和免疫荧光检测技术研究精子携带的HBV基因在胚胎细胞中的复制与表达。
2.
BACKGROUND&AIM: To explore the feasibility of hepatitis B virus(HBV)vertical transmission via male germ line,the replication and expression of sperm-mediated HBV DNA in mouse embryo were studied.
背景与目的:研究乙型肝炎病毒(HepatitisBvirus,HBV)DNA在早期胚胎中能否复制与表达,探讨HBV经雄性生殖细胞垂直传递的可能性。
补充资料:复制质粒
分子式:
CAS号:
性质:含有一个复制起始点,并在宿主细胞中能自主复制和稳定遗传的质粒。
CAS号:
性质:含有一个复制起始点,并在宿主细胞中能自主复制和稳定遗传的质粒。
说明:补充资料仅用于学习参考,请勿用于其它任何用途。
参考词条