Conclusion:This method is a quick,simple and accurate one for sequencing DNA,especially for short fragment,and it is suitable for gene diagnosis in clinical laboratory.

To explore the relationship between the structure and function of cell toxin gelonin as the bullet of immunoconjugate to specially kill and wound target cells, truncated gelonins deleted at N-terminal region Gly, Leu, Asp and/or C-terminal Asp, Lys, Asp, Pro, Lys were designed on the basis of its gene sequence and three dimentional molecular conformation.