1) human microvascular endothelial cells
人微血管内皮细胞
1.
Methods The PCR products of t-PA target gene and β-actin housekeeping gene were obtained using RT-PCR after the total RNA was extracted from human microvascular endothelial cells(HMEC-1).
目的建立实时RT-PCR检测人微血管内皮细胞组织型纤溶酶原激活物(t-PA)mRNA的表达。
2) HMEC-1
人微血管内皮细胞
1.
The purity of rhAS was identified by SDS-PAGE,and the activity was performed on the model of human micro-vascular endothelial cell line(HMEC-1) and chick chorioallantoic membrane.
coli,M15/pQE/AS)的IPTG诱导条件及包涵体复性纯化条件进行优化,产物以SDS-PAGE鉴定纯度,在人微血管内皮细胞模型及鸡胚尿囊膜模型上鉴定其生物学活性。
3) microvascular endothelial cells of human skin
人皮肤微血管内皮细胞
1.
The expression of ActivinA gene in microvascular endothelial cells of hum an skin and the effect of ActivinA protein on the cell proliferation Experimental study on transfecting ActivinA gene into the microvascular endothelial cells of human skin by catio
激活素A在人皮肤微血管内皮细胞中的表达及对其增殖的抑制作用
4) human brain microvascular endothelial cell
人脑微血管内皮细胞
1.
Methods Human recommbinant MIP-1α(rhMIP-1α) was incubated with human brain microvascular endothelial cell (HBMEC) monolayer and the permeability of HBMEC monolayer was observed with immunofluorescence, TEER and HRP flux methods.
方法以重组人MIP-1α直接作用于人脑微血管内皮细胞(HBMEC),免疫荧光方法检测紧密连接蛋白ZO-1的分布变化、跨内皮细胞电阻、HRP穿过HBMEC单层的改变、HBMEC细胞CC趋化因子受体5(CCR5)的表达,以及MIP-1α中和抗体和分泌MIP-1α的模式细胞(6T-CEM)与HBMEC单层共同温育时ZO-1的分布变化。
2.
We have showed the stronger ability to migrate through human brain microvascular endothelial cells (HBMECs) and a significantly higher macrophage inflammatory protein-1α (MIP-1α) expression in peripheral T lymphocytes of AD patients than age-matched healthy subjects.
我们研究曾发现,AD病人外周血T淋巴细胞穿过人脑微血管内皮细胞(HBMEC)单层能力高于同龄正常人,其T淋巴细胞巨噬细胞炎症蛋白1α(MIP-1α)表达明显增高。
3.
To reveal the expression of vascular endothelial growth factor (VEGF) under conditions that cause endoplasmic reticulum (ER) stress, human brain microvascular endothelial cells (HBMEC) were treated with tunicamycin at different concentration in vitro.
为观察内质网应激条件下血管内皮细胞生长因子的表达情况,用不同浓度的衣霉素处理体外培养的人脑微血管内皮细胞,建立内质网应激模型,采用RT-PCR、蛋白质免疫印迹以及免疫细胞化学的方法检测了细胞内血管内皮细胞生长因子的表达。
5) human pulmonary microvascular endothelial cells
人肺微血管内皮细胞
1.
Effects of direct stimulation by angiotensin-Ⅱon activation of nuclear factor kappa B of human pulmonary microvascular endothelial cells;
血管紧张素Ⅱ对人肺微血管内皮细胞核因子-κB的活化作用
6) human brain microvascular endothelial cells
人脑微血管内皮细胞
1.
Influence of ox-LDL on activities of human brain microvascular endothelial cells and expressions of adhesion molecules;
ox-LDL对人脑微血管内皮细胞活性及黏附分子表达的影响
2.
Phase contrast microscopy was used to observed the morpho- logical changes of human brain microvascular endothelial cells (HBMEC), and Hoechst 33258.
04kb)克隆到pQE30表达载体,构建表达产物为N末端带有6个组氨酸(His)序列的yijP汇合蛋白,以M15(pREP4)为受体菌,大量表达(His)6-yijP汇合蛋白,利用Ni-NTA亲和层析纯化汇合蛋白,将经透析法复性的一定浓度的(His)6-yijP蛋白加入到体外培养的人脑微血管内皮细胞中,结果显示yijP蛋白对人脑微血管内皮细胞有较强的细胞毒作用:在相差显微镜下可观察到细胞皱缩、胞膜呈泡状膨出,随着时间延长细胞逐渐脱落;荧光显微镜下可见细胞核呈现为致密团块状或圆形浓染颗粒状,呈凋亡样改变;DNA琼脂糖凝胶电泳可见DNA阶梯状条带;流式细胞仪显示在正常二倍体峰之前出现一个亚二倍体峰;Western印迹可检测到caspase-3的活性片段。
补充资料：肝婴儿血管内皮细胞瘤
肝婴儿血管内皮细胞瘤
hepatic infantile hemangioendothelioma
类似毛细血管瘤的肝血管畸形疾病。有一定时期的自限病程，分增生、成熟、退化等几个阶段，如无危及生命的并发症，病变则最终消失。本病多在6个月内的婴儿得以确诊，女性多于男性；偶有成年病例的报道。常以肝大和/或腹部包块为主要临床表现，多同时伴有皮肤毛细血管瘤；部分患者因有动静脉瘘而出现充血性心力衰竭。有症状者约2/3会死于该病，死因有充血性心力衰竭、肝功能衰竭或肿瘤破裂出血。单发性者有手术切除机会；多发性则常不能手术切除，可选择肝动脉结扎或栓塞、放疗和皮质激素治疗等。个别可伴发血管肉瘤。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条