G banding karyotype analysis of peripheral lymphocytes in 217 patients with azoospermia or severe oligospermia were performed and the Y-chromosome AZFc region from seven cases with Y chromosome abnormality was amplified by polymerase chain reaction(PCR).

Objective: To study the relationship between the single nucleotide plymorpism(SNP) rs551373(G>T) in gonadotropin-regulated testicular RNA helicase(GRTH) gene and severe oligospermia.

Methods: FASL-844 polymorphism was genotyped by polymerase chain reaction and restriction fragment length polymorphism(PCR-RFLP) in 184 infertile patients with idiopathic azoospermia or severe oligozoospermia 236 normal fertile male controls.

AIM: To discuss the relationship between the azoopermia factor (AZF) and the level of nitric oxide (NO) in patients with unobstructed azoospermia and severe oligozoospermia.

Among them,lost of segment in different area was discovered in 4 cases of azoospermia and severe oligozoospermia.

BACKGRONUD & AIM: To investigate the relationship between spermatogenesis disorder and genetic defects of patients with azoospermia or severe oligospermia.

Methods Karyotypes were analyzed by chromosome G-banding in 415 infertile patients with idiopathic azoospermia or severe oligozoospermia.

To investigate the distribution of DAZ gene copy deletions among Chinese men, we analyzed DAZ gene deletions by multiplex polymerase chain reaction (multi-PCR) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 128 infertile patients with severe oligozoospermia selected as semen donors for intracytoplasmic sperm injection (ICSI) and 287 normospermic men.

To investigate the distribution of the two genetic defects in Chinese patients with azoospermia or severe oligozoospermia, karyotype analysis by G-banding was carried out in 358 idiopathic infertile men, including 256 patients with azoospermia and 102 patients with severe oligozoospermia,and screening of AZF region microdeletion of Y chromosome by .