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1)  Peripheral Blood Mononuclear cells
外周血单一核细胞
1.
Expression of the receptors of CXCR1 and CXCR2 mRNA in peripheral blood mononuclear cells in patients with psoriasis;
银屑病患者外周血单一核细胞中趋化因子受体CXCR1及CXCR2 mRNA的表达
2.
Expressions of Fas, FasL and bcl-2 on peripheral blood mononuclear cells in patients with polymyositis and dermatomyositis;
多发性肌炎和皮肌炎患者外周血单一核细胞Fas、FasL和bcl-2分子的表达
3.
Objective To investigate the effects of Aloe on interferonγ(IFN-γ)production by peripheral blood mononuclear cells(PBMCs )from patients with psoriasis.
目的探讨芦荟对银屑病患者外周血单一核细胞(PBMCs)产生干扰素-γ(IFN-γ)的影响。
2)  peripheral blood mononuclear cell
外周血单一核细胞
1.
Objective:To study the state of p16 gene DNA methylation on peripheral blood mononuclear cell (PBMC) and polymorphonuclear leukocyte (PMNL) in the patients with systemic lupus erythematosus (SLE) and their significances in the pathogenesis of the disease.
目的:研究系统性红斑狼疮(SLE)患者外周血单一核细胞(PBMC)及中性粒细胞(PMNL)的p16基因甲基化状态,探讨其在SLE发病中的作用。
2.
To detect and type HPV DNA in peripheral blood mononuclear cells ( PBMCs) and in local lesions of patients with condyloma acuminata ( CA).
检测尖锐湿疣(condyloma acuminata,CA)病人外周血及局部皮损组织中的人乳头瘤病毒(human papillomavirus,HPV)DNA存在情况,并进行HPV分型,探讨外周血单一核细胞(peripheral blood mononuclear cells,PBMCs)中检测到的HPV与局部皮损组织中HPV感染的关系。
3.
Objective: To assess the expressions of chemokine receptors CX3CR mRNA on peripheral blood mononuclear cell(PBMC) with active or inactive systemic lupus erythematosus(SLE).
目的:探讨趋化因子受体CX3CR1在系统性红斑狼疮(SLE)患者外周血单一核细胞(PBMC)中的表达,及其与疾病活动的相关性。
3)  peripheral blood mononuclear cell
外周血单核细胞
1.
Construction of cDNA library of peripheral blood mononuclear cells of pigs inoculated with highly pathogenic porcine reproductive and respiratory syndrome virus
高致病性猪生殖与呼吸综合征病毒感染猪外周血单核细胞cDNA文库的构建
2.
Expression of MCP-1 in peripheral blood mononuclear cells from patients with Alzheimer’s disease
阿尔茨海默病患者外周血单核细胞MCP-1的表达
3.
Aim:To explore the expression of heme oxygenase 1(HO 1) in the peripheral blood mononuclear cell (PBMC) and relationship to ventilatory function in asthmatic patients.
目的 :探讨血红素氧合酶 1(HO 1)在哮喘患者外周血单核细胞 (PBMC)的表达及与肺通气功能的关系。
4)  PBMC
外周血单核细胞
1.
Regulation effect of recombinant interferon-γ(rIFN-γ) on expression of IL-4 and IL-10 mRNA in peripheral blood mononuclear cells(PBMC) of asthmatic patients;
重组干扰素-γ对哮喘患者外周血单核细胞IL-4和IL-10 mRNA表达的调节作用
2.
Methods:Obtained heparinized venous blood of RTRs before Tx,then isolated PBMC by Ficoll density-gradient centrifugation.
目的:肾移植术后应用Calcineurin抑制剂需常规监测血清药物浓度,然而药物浓度并不能完全反应患者的免疫功能抑制状态,且药物的个体反应性差异较大,本研究旨在通过外周血单核细胞(PBMC)活性检测反应患者对Calcineurin抑制剂的敏感性。
3.
【Objective】 The study was to explore the effects of endometrial stroma cells(ESC) on proliferation and secretion activity of PBMC and PBL in goat via the way of paracrine.
【目的】探索山羊子宫内膜基质细胞(ESC)培养上清,对山羊外周血单核细胞(PBMC)和外周血淋巴细胞(PBL)的转化与分泌活性的影响。
5)  peripheral blood mononuclear cells
外周血单核细胞
1.
Activation of NF-κB induced by coronary stent placement in peripheral blood mononuclear cells;
经皮冠状动脉内支架植入术对外周血单核细胞NF-κB活性的影响
2.
Endotoxin regulates activity of human β-glucuronidase in peripheral blood mononuclear cells in vitro;
内毒素诱导外周血单核细胞释放β-葡萄糖苷酸酶的体外研究
3.
Expression of human triggering receptor expressed on myeloid cells 1 in peripheral blood mononuclear cells of patients with acute obstructive suppurative cholangitis
人髓样细胞触发性受体-1在急性梗阻性化脓性胆管炎外周血单核细胞中的表达
6)  peripheral blood monocytes
外周血单核细胞
1.
In the cultured cells of peripheral blood monocytes,LC-MS was used to assay the intracellular concentrations of streptomycin thereby investigating the in vitro penetrability of streptomycin before and after nanoparticlized.
方法:以氰基丙烯酸正丁酯为聚合材料,通过乳化聚合法制备链霉素纳米粒,以人外周血单核细胞为体外细胞模型,采用高效液相色谱-质谱法检测链霉素的胞内浓度,对链霉素纳米粒和链霉素的跨膜转运情况进行对比考察。
2.
OBJECTIVE To investigate the effects of tanshinone on peripheral blood monocytes(PBMC)activation in patients with essential hypertension(EH)during early stage of the diseased course.
目的研究丹参酮对原发性高血压(EH)病人病程早期外周血单核细胞(PBMC)激活的作用。
3.
Methods Peripheral blood monocytes from healthy donors were treated with rhGM CSF of 100 ng/ml、rhGM CSF of 100ng/ml plus IL 4 of 50ng/ml and rhGM CSF plus A 23187 100ng/ml each, respectively.
目的 探讨钙载体 (calciumionophore ,CI)A2 3 187对正常人外周血单核细胞来源的树突状细胞(Dendriticcell,DC)形态、表面分子表达及免疫功能的影响。
补充资料:人体外周血淋巴细胞培养

  
  人体外周血淋巴细胞培养
  

  诊法。为 检查患者染色体最简便的细胞培养技术。先于无菌条件下制备RPMI1640 或TC199等常用培养基,加适量小牛血清和PHA(植物血球凝集 素),夏季操作时最好加适量青、链霉素,调pH值为7.0~7.2左右,4℃条件下保存6个月 。检测时,以500IU/ml肝素湿润注射器,抽取患者静脉血1.5~2ml(或取耳垂、指尖或足 跟等处微量血),每份培养基(5ml)内种血0.2~0.3ml,37℃温箱内静置培养72小时,收获 前3~4小时加秋水仙素(或秋水仙胺),最终浓度为0.02μg/ml,使细胞分裂终止于中期。 取出培养物行离心、低渗和固定等一系列细胞学操作获得染色体标本,以Giemsa染色后镜检 ,可作染色体标本的普通分析。现各实验室一般将染色体行G显带或必要的其他多种显带, 以较精确地对染色体标本进行分析。
  
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