Objective To investigate changes in the morphology and expression of collagen typeⅠand Ⅱ and core protein of aggrecan of chondrocytes during the in vitro culture, and to select the best time of chondrocytes transplant in the tissue engineering.

Then X-gal staining,And Group 1,RT-PCR for TIMP-3 and aggrecan core protein,TUNEL staining,immunohistochemical staining for TIMP-3 and type I! collagen and Safranin O-Fast green staining was carried out to assess the effects of RadTIMP-3 transfection.

The aggrecanases and their activity regulation

Effects of nivalenol and selenium on the metabolism of aggrecan in chondrocytes;

Protective effect of selenium against T-2 toxin-induced inhibition of chondrocyte aggrecan and collagen Ⅱ synthesis;

The mRNA levels of collagen II,aggrecan,sox9,Indian hedgehog(ihh),parathyroid hormone-related protein(PTHrP),bone morphogenetic protein(BMP)-4 and wnt5a were examined with RT-PCR,and protein levels of collagen II,aggrecan were examined with immunohistochemistry and toluidine blue staining.

Effects of JianGu Capsules on Proteoglycan Content of Articular Cartilage in Model Rabbit with Osteoarthritis;

Study of isolation and identification of chondroitin sulfate proteoglycan in pliose antler;

An Experimental Study on Changes of Proteoglycan of Cervical Cartilage Endplate under Long Term Abnormal Stress Conditon in Rabbit;

Experimental Study on the Relationship between Heparin Sulfate Proteoglycans of Endothelial Cells and Cell Adhesion;

Effects of coriolus versicolor polysaccharides B on macrophage surface chondroitin sulfate-proteoglycans binding oxidized low density lipoprotein