TD-PCR,TD-PCR,在线英语词典,英文翻译,专业英语

1) TD-PCR

TD-PCR

The TD-PCR and Phylogenetic Analysis of Bacillus licheniformis 16S rDNA;

地衣芽孢杆菌16S rRNA基因的TD-PCR扩增及系统发育分析

Human bactericidal/permeability-increasing protein(hBPI)cDNA was amplified by reverse transcription(RT)and touchdown PCR(TD-PCR)from blood stem cells collected from healthy human of Uygur nationality in Xinjiang Uygur Autonomous Region of China, and then was subcloned into pEGFP-N1 vector,hBPI cDNA sequence consists of 1,464bp.

从新疆维吾尔族健康人造血干细胞中提取总RNA,用反转录(reverse transcription,RT)和降落PCR (touchdown PCR,TD-PCR)相结合的方法扩增人细菌透性增加蛋白(human bactericidal/permeability-increasing protein,hBPI)的cDNA,将其克隆到pEGFP-N1载体上并进行DNA序列测定。

The sequences of 16S rRNA gene were amplified by TD-PCR with the bacterium universal primers and the purified PCR products were directly sequenced.

菌株经纯化培养,以改良CTAB法提取总DNA,采用细菌16S rRNA通用引物、TD-PCR方法(touchdown-PCR)进行16S rRNA基因序列扩增,PCR产物纯化后直接进行序列测定,序列经人工校对后用Clustal X进行比对分析,最后用MEGA3。

2) TD-PCR

降落PCR

Touchdown PCR (TD-PCR) was done and among the products, 19 fragments with 800~1 000 bp in length were selected for sequence analysis.

富集培养胞外蛋白酶产生菌并提取了12个总DNA样品,分别用每对引物在降落PCR (TouchdownPCR, TD-PCR)条件下进行蛋白酶编码序列的扩增。

3) TD-PCR

降落聚合酶链反应

4) TD PCR

降落PCR

TD PCR——AN AMELIORATIVE PCR FOR AMPLIFICATION OF TARGET GENES;

一种优化的PCR方法——降落PCR扩增目的基因

Target genes were amplified with the TD PCR method,two orthogonal experiments were designed to screen out optimal concentrations of various components of the PRC reaction system,while to explore annealing time,extending time and cycling frequency.

采用降落PCR方法扩增目的基因,设计两次正交试验对PCR反应体系中的各组分浓度进行筛选,同时对退火时间、延伸时间及循环次数进行摸索。

Using the primers, the PVⅢ gene was amplified by touchdown PCR (TD PCR).

1kb左右的引物 ,采取降落PCR对EDSV gDNA进行扩增。

5) AtSUC99 TD PCR

AtSUC99 TD PCR

6) Time domain polymerase chain reaction (TD-PCR)

时域聚合酶链式反应

补充资料：TD50

分子式：
CAS号：

性质：药物引起半数实验动物中毒的剂量。

说明：补充资料仅用于学习参考，请勿用于其它任何用途。

首页 \| 翻译 \| 影视 \| 论坛 \| 索引

1) TD-PCR TD-PCR 1. The TD-PCR and Phylogenetic Analysis of Bacillus licheniformis 16S rDNA; 地衣芽孢杆菌16S rRNA基因的TD-PCR扩增及系统发育分析 2. Human bactericidal/permeability-increasing protein(hBPI)cDNA was amplified by reverse transcription(RT)and touchdown PCR(TD-PCR)from blood stem cells collected from healthy human of Uygur nationality in Xinjiang Uygur Autonomous Region of China, and then was subcloned into pEGFP-N1 vector,hBPI cDNA sequence consists of 1,464bp. 从新疆维吾尔族健康人造血干细胞中提取总RNA,用反转录(reverse transcription,RT)和降落PCR (touchdown PCR,TD-PCR)相结合的方法扩增人细菌透性增加蛋白(human bactericidal/permeability-increasing protein,hBPI)的cDNA,将其克隆到pEGFP-N1载体上并进行DNA序列测定。 3. The sequences of 16S rRNA gene were amplified by TD-PCR with the bacterium universal primers and the purified PCR products were directly sequenced. 菌株经纯化培养,以改良CTAB法提取总DNA,采用细菌16S rRNA通用引物、TD-PCR方法(touchdown-PCR)进行16S rRNA基因序列扩增,PCR产物纯化后直接进行序列测定,序列经人工校对后用Clustal X进行比对分析,最后用MEGA3。 2) TD-PCR 降落PCR 1. Touchdown PCR (TD-PCR) was done and among the products, 19 fragments with 800~1 000 bp in length were selected for sequence analysis. 富集培养胞外蛋白酶产生菌并提取了12个总DNA样品,分别用每对引物在降落PCR (TouchdownPCR, TD-PCR)条件下进行蛋白酶编码序列的扩增。 3) TD-PCR 降落聚合酶链反应 4) TD PCR 降落PCR 1. TD PCR——AN AMELIORATIVE PCR FOR AMPLIFICATION OF TARGET GENES; 一种优化的PCR方法——降落PCR扩增目的基因 2. Target genes were amplified with the TD PCR method,two orthogonal experiments were designed to screen out optimal concentrations of various components of the PRC reaction system,while to explore annealing time,extending time and cycling frequency. 采用降落PCR方法扩增目的基因,设计两次正交试验对PCR反应体系中的各组分浓度进行筛选,同时对退火时间、延伸时间及循环次数进行摸索。 3. Using the primers, the PVⅢ gene was amplified by touchdown PCR (TD PCR). 1kb左右的引物 ,采取降落PCR对EDSV gDNA进行扩增。 5) AtSUC99 TD PCR AtSUC99 TD PCR 6) Time domain polymerase chain reaction (TD-PCR) 时域聚合酶链式反应补充资料：TD50 分子式： CAS号：性质：药物引起半数实验动物中毒的剂量。说明：补充资料仅用于学习参考，请勿用于其它任何用途。
©2008 dictall.com