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1)  esat6-ppe68 fusion gene
esat6-ppe68融合基因
1.
Objective To construct esat6-ppe68 fusion gene and its prokaryotic expression vector for expression in E.
目的构建结核分支杆菌esat6-ppe68融合基因及其原核表达载体,在大肠杆菌中表达融合蛋白ESAT6-PPE68。
2)  cfp10-esat6 fusion gene
cfp10-esat6融合基因
1.
To begin with,we constructed cfp10-esat6 fusion gene and its prokaryotic expression vector and had it express in E.
构建结核分枝杆菌cfp10-esat6融合基因及其原核表达载体,在大肠杆菌中表达融合蛋白CFP10-ESAT6。
3)  Lhp-esat6 fusion gene
lhp-esat6融合基因
4)  esat6-rpfD fused gene
esat6-rpfD融合基因
1.
Objective:To construct prokaryotic expression vector of Mycobacterium tuberculosis esat6-rpfD fused gene and express and purify the fusion protein ESAT6-RpfD in E.
结论:构建了esat6-rpfD融合基因原核表达载体,并在大肠杆菌中表达并纯化得到ESAT6-RpfD融合蛋白。
5)  esat6 gene
esat6基因
1.
cfp10 was inserted into the cloning vector pGEM-T-easy with esat6 gene.
方法:以结核杆菌H37Rv基因组DNA为模板,克隆cfp10基因,将其插入到pGEM-T-easy载体后与esat6基因连接,构建含有esat6和cfp10融合基因的重组载体pGEM-e6c10。
2.
Methods: ESAT6 gene was amplified by PCR with specific primer from genome of Mycobacterium tuberculosis (MTB)H37Rv.
目的:构建结核分枝杆菌(MTb)ESAT6基因表达载体并在戈登链球菌GP251中进行分泌表达。
3.
To construct expressing vector carrying esat6 gene and express this protein in Streptococcus gor-donii GP251.
为了构建结核分枝杆菌(MTb)esat6基因表达载体并在戈登链球菌GP251中进行分泌表达,以结核杆菌H37Rv基因组DNA为模板扩增esat6基因,将esat6基因TA克隆到pMD18-T,构建pMD18-esat6重组载体。
6)  recombinant ESAT6-PPE68
rESAT6-PPE68融合蛋白
补充资料:wet strength agent PPE
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性质:琥珀色透明液体。固含量20%±0.5%。粘度0.03~0.04帕·秒(25℃)。系聚酰胺-多胺-环氧氯丙烷阳离子型热固型树脂。在造纸过程中用作湿强剂,湿强效果好。适合中性或碱性造纸,对填料及细小纤维有助留作用,并有助滤作用。为无毒非甲醛型树脂,其废纸回收制浆比较容易。适用于特种工业用纸和包装纸、彩色照片原纸、茶叶滤纸、绝缘板纸等。由己二酸、多乙烯多胺、环氧氯丙烷等反应制得。

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