Induction and expression of T4 lysozyme gene in Pichia pastoris;

The molecular packing was analyzed with 10 different crystal forms of T4 lysozyme, where there is only one protein molecule in an asymmetric unit.

In order to efficiently recover recombinant proteins, a temperature sensitive lytic system was constructed on the basis of the feature that T4 lysozyme disrupts the bacteria through cutting specific bond in the peptidoglycan layer of cell wall.

Cap gene of PCV2 was amplified by PCR and cloned into the expressing plasmid pSOC,where Cap gene was fused to 3 end of T4 phage small outer capsid gene encoding SOC protein.

NP gene of AIV was amplified by PCR and cloned into the expressing plasmid pSOC, where NP gene was fused to 3′end of T4 phage small outer capsid gene encoding SOC protein.

The recombinant plasmid was transformed into Escherichia coli 2(E2),the host bacteria of T4 phage.

Construction and identification of foot-and-mouth disease virus entire capsid protein precursor(P1) gene recombinant T4 bacteriophage;

T4 bacteriophage surface display of foot-and-mouth disease virus 3 C protease;

Display of globular domain of HN protein of Newcastle disease virus on bacteriophage T4 capsid surface;

Fresh-Keeping Experiment of Chilled Meat by Small Tray Package with Lysozyme,Nisin and GNa Solution;

Phase behaviors of the interaction between the anionic surfactants and lysozyme;

Effect of betaine on unfolding thermodynamics and refolding kinetics of lysozyme;