1) Transcriptional activation activity
转录激活活性
1.
Transcriptional activation activity of variant was evaluated through methods .
方法:运用RT-nested PCR扩增人糖皮质激素受体(GR)基因不含编码LBD的cDNA序列,将PCR产物定向克隆至pEGFP-N2质粒载体,测序筛选编码正确的重组质粒;荧光显微镜观察GR突变体重组质粒转染人巨噬细胞株U937后的表达情况;相对荧光素酶法检测转染后细胞GR转录激活活性。
2.
The transcriptional activation activity of variant was evaluated by the relative activity of luciferase.
方法运用RT-nested PCR扩增糖皮质激素受体不含有编码LBD(ligand b ind ing dom ain)的cDNA序列,将PCR产物定向克隆至pEGFP-N2质粒载体,测序筛选重组质粒;荧光显微镜观察重组质粒转染表达情况;相对荧光素酶法检测GR转录激活活性。
3.
The transcriptional activation activity of GR was evaluated by the method of relative activity of luciferase.
方法 将GR荧光表达质粒pGFP GR转染大鼠肺泡巨噬细胞,经LPS和SNP作用后,荧光显微镜观察质粒表达产物GFP GR核移位情况;相对荧光素酶法检测GR转录激活活性;EMSA检测检测细胞NF κB活性。
2) transcriptional activation
转录激活活性
1.
Results showed that RDV P9 performed the transcriptional activation in yeast.
将RDV微核心蛋白基因S9克隆到酵母表达载体pGBKT7中,转入AH109酵母细胞,转化子可以在SD/Trp-His-Ade-多营养缺陷固体培养基上正常生长,证明RDV P9在酵母中具有转录激活活性,运用β-半乳糖苷酶的活性分析对重组质粒转化子的转录激活程度做了定量分析,发现与正对照相比,转化pGBK-S9的酵母菌中β-半乳糖苷酶活性可达到正对照的40%以上。
2.
In order to study the transcriptional activation activity of PC-1,firstly, the yeast two-hybrid system was used and the whole length as well as various regions of PC-1cDNA were cloned into the expression vector pAS2-1 and transfected into the yeast cell line CG-1945 respectively, the result of the reporter genes.
lacZ和His3报告基因激活的检测结果表明 ,该分子具有转录激活活性并将该活性定位于N端的 4 6个氨基酸区域 。
3) transcription activation
转录激活
1.
The chromatin remodeling and histone acetylation were associated with transcription activation.
真核基因转录激活调控是在启动子、增强子等顺式调控元件和转录因子、激活因子以及 RNA聚合酶 II(Pol II)等调控蛋白的参与下协同进行的。
2.
In this study, a mammalian transcription activation system was constructed by using DNA binding domain(DBD) of GAL4 and luciferase reporter gene with DBD binding sequence, and used for mapping of FHL2 transcription activation domain.
利用GAL4转录因子中的DNA结合结构域 (DBD)和含有与DBD结合序列的荧光素酶报告基因(GAL4 LUC)构建了哺乳动物细胞转录激活系统 ,并利用该系统定位了FHL2的转录激活结构域。
4) Transcriptional activation
转录激活
1.
Objective:To construct Smads transcriptional activation system for studying the function of Smads.
目的:构建Smads转录激活检测系统,以研究Smad2、Smad3、Smad4的功能。
2.
Methods: As EDAG has the activity of transcriptional activation, we set up a new model for large scaled screening small molecular compounds on 96-well plate by luciferase r.
方法:利用荧光素酶报道基因的实验方法,将EDAG的转录激活活性量化,建立大规模筛选活性小分子化合物的模型,筛选小分子化合物库(约1000个)。
5) Transcriptional activity
转录活性
1.
Clone of human fatty acid synthase promoter and analysis of its transcriptional activity in tumor cells;
人脂肪酸合成酶FAS启动子的克隆及其在几种肿瘤细胞中的转录活性分析
2.
Construction of BMP-2 expression vector and study on its transcriptional activity.;
BMP-2表达载体的构建及其转录活性测定
3.
Molecular cloning,sequence analysis and transcriptional activity determination of cytochrome P450 gene CYP18A1 in the silkworm,Bombyx mori;
家蚕P450基因CYP18A1的克隆、序列分析及转录活性
6) transcription activity
转录活性
1.
The transcription activity of DNA regulation of TIAR through the shuttle mechanism between DNA and RNA;
TIAR通过在DNA和RNA之间的穿梭机制调控DNA的转录活性
2.
The distribution of global helical twists for bacterial promoters and transcription activity;
细菌启动子间隔序列的螺旋扭角分布与转录活性
3.
Effect of propofol on the transcription activity of endothelial nitric oxide synthase gene promoter in human umbilic vein endothelial cells induced by lipopolysaccharide;
异丙酚对脂多糖诱导人脐静脉内皮细胞内皮源型一氧化氮合酶基因启动子转录活性的影响
补充资料：活性粘土,活性白土
CAS：68333-91-5
中文名称：活性粘土；活性粘土,活性白土
英文名称：Bentonite, lime-activated；Activated clay；Bentonite,lime-activated；active clay
中文名称：活性粘土；活性粘土,活性白土
英文名称：Bentonite, lime-activated；Activated clay；Bentonite,lime-activated；active clay
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条