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1)  double in situ hybridization
双重原位杂交
2)  in situ double hybridization
原位双杂交
1.
In other to clarify the frequency of p16INK4 gene inactivation and its relationship with retinoblastoma (Rb) gene expression in non-small cell lung cancer, in situ double hybridization was established with Bio-labelled Rb cDNA probe and Dig-lablled p16INK4 cDNA probe, and immunohistochemistry was carried out with polyclone rabbit antibodies to human p16INK4 and Rb protein.
采用ｍＲＮＡ原位双杂交和免疫组织化学方法对３１例非小细胞肺癌组织进行ｐ１６ＩＮＫ４、Ｒｂ基因表达水平及其相关性的研究。
3)  in situ hybridization
原位杂交
1.
Expression of Pax3 gene in Chick Embryo spinal cord analyzed with the improved in situ hybridization;
应用改良的原位杂交方法分析Pax3基因在鸡胚脊髓中的表达
2.
Complex chromosomal aberrations detected by fluorescence in situ hybridization and their clinical significance in patients with myelodysplastic syndrome;
荧光原位杂交检测骨髓增生异常综合征患者染色体复杂核型变化及临床意义的研究
3.
Establishing of in situ hybridization method for the expression of dentin phosphoprotein mRNA;
牙本质磷蛋白mRNA原位杂交方法的建立
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4)  in-situ hybridization
原位杂交
1.
A whole-mount in-situ hybridization study on expression profile of mCcd1 during mouse embryonic development;
整体原位杂交研究mCcd1在小鼠胚胎发育过程中的表达
2.
Methods: In-situ hybridization and immunohistochemistry were used to detect the expressions of CyclinE mRNA and CyclinE protein in 40 cases of colon carcinoma in various stages,and 10 colon adenoma,and 10 normal colon mucosa.
方法:采用原位杂交方法和免疫组化S-P法对40例结肠癌1、0例结肠腺瘤和10例结肠正常粘膜组织进行CyclinE mRNA和CyclinE蛋白的检测,同时结合临床病理资料进行分析。
3.
The hSAMP32 mRNA expression was detected by in-situ hybridization.
原位杂交检测hSAMP32mRNA在3组细胞中的表达。
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5)  Immunohistochemistry
原位杂交
1.
Immunohistochemistry and in situ hybridization technique for undecalcified bone sections of human;
人类不脱钙骨组织的免疫组化与原位杂交
2.
The IgA nephropathy rats model were made by the method of oral BSA and intravenous SEB,the mRNA and protein expression of TGF-β_1 was measured by in-situ hybridisation and immunohistochemistry.
采用口服牛血清白蛋白及静注葡萄糖球菌肠毒素制成小鼠IgA肾病模型,免疫组化和原位杂交检测肾小球转化生长因子β1(TGFβ-1)蛋白和mRNA。
3.
Methods:Expressional level of nm23mRNA,p16 was detected in 72 human lung cancer samples and 23 normal tissues adjacent to cancer respectively,and their correlation with lymph node metastasis and the degree of cell differentiation was studied by immunohistochemistry (IHC) and in situ hybridization (ISH) methods on tissue microarray.
方法 :应用组织芯片技术 ,通过原位杂交和免疫组织化学方法对 72例人肺癌组织和 2 3例癌旁正常肺组织中nm2 3mRNA和p16的表达水平及其与肺癌分化程度、淋巴结转移的关系进行研究。
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6)  ISH [英][ɪʃ]  [美][ɪʃ]
原位杂交
1.
A Convenient Non-radio ISH Method for Detection of Pancreatic Elastase Ⅱ mRNA;
一种简便的非放射性原位杂交法检测胰腺Ⅱ型弹性蛋白酶mRNA
2.
Using ISH to Study the Expression and Distribution of FcRn in the Small Intestine of the Piglet;
利用原位杂交技术研究仔猪小肠FcRn表达及分布规律
3.
Methods :ISH were used to determine the effect of Ganoderma appanatum polysaccharides GF on N-ras 、C-myc mRNA in mouse HepA tumor.
方法:应用地高辛标记探针原位杂交技术,检测树舌多糖GF对HepA瘤细胞中癌基因N-ras、C-myc mRNA丰度的影响。
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补充资料：荧光原位杂交


荧光原位杂交


是以荧光素标记取代核素标记而形成的一种新的原位杂交方法。利用已知碱基序列的非核素标记的核酸探针，依据碱基配对原理，通过免疫细胞化学检测体系，在组织切片、细胞间期核或染色体等标本上，进行DNA定性、定位及定量分析。本法快速、安全、灵敏度高，特异性强。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
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