1) Immunofluorescence
[英][i'mju:nəu,fluə'resns] [美][,ɪmjəno,flʊə'rɛsəns]
免疫荧光法
1.
METHODS: SP immunohistochemical staining and immunofluorescence were performed in paraformaldehyde fixed hippocampal sections.
方法 :在多聚甲醛固定的海马脑薄片上 ,用免疫组化SP法和免疫荧光法观察 p CREB在正常成年大鼠海马结构的表达与分布 。
2.
METHODS Indirect immunofluorescence method and MTT method were used to test the expression of human IL-6 receptor on R2 cells and the reaction of R2 cells to recombinant human IL-6(rhIL-6).
方法分别用间接免疫荧光法和MTT检测法检测了R2细胞的人IL 6受体蛋白表达水平和对重组人白介素 6(rhIL 6 )的反应性。
3.
Methods Immunohistochemistry was used to detect expression of ABCG2 in 40 cases of transitional cell carcinoma of bladder with different pathology grades(12 cases were Grade Ⅰ,18 cases were Grade Ⅱ,10 cases were Grade Ⅲ)and 15 normal bladder tissues,while immunofluorescence was used to detect expression of ABCG2 in BIU-87 cell line.
方法对40例膀胱移行细胞癌组织(其中Ⅰ级12例,Ⅱ级18例,Ⅲ级10例)和15例正常膀胱组织采用免疫组织化学法检测,对人膀胱癌细胞株BIU-87采用免疫荧光法检测ABCG2蛋白的表达,显微镜观察并对相关图片进行光密度分析。
2) immunofluorescence method
免疫荧光法
1.
Expression of Survivin in leukemia by Western blot analysis and immunofluorescence method;
蛋白印迹法与免疫荧光法检测白血病患者Survivin表达
2.
RuBPCase in leaf blade transection of typical C 3, C 4 plants and Hevea brasiliensis was located by direct immunofluorescence method.
采用直接免疫荧光法对典型的 C3植物水稻、C4 植物甘蔗和巴西橡胶树等进行了 Ru BP羧化酶的定位 。
3.
Rabbit antiserum raised purified ribulose 1,5 bisphosphate carboxylase (RuBPCase) from tobacco was used to locate RuBPCase in leaf blade transection of classical C 3, C 4 plants and Coffea arabica by direct immunofluorescence method.
采用直接免疫荧光法对典型 C3植物水稻、C4 植物甘蔗和小粒种咖啡等进行了 Ru BP羧化酶的组织化学定位 。
3) Indirect Immunity fluorescence method
免疫荧光免疫法
4) Indirect immunofluorescence
间接免疫荧光法
1.
Application of indirect immunofluorescence(IFL) in the detect of antibody to eberthella typhi and bacillus paratyphosus;
间接免疫荧光法(IFL)测抗伤寒、副伤寒沙门菌IgM抗体
2.
Methods: The ANA was detected with indirect immunofluorescence(IIF) and protein chip respectively,and the other six autoantibodies including anti-dsDNA,anti-Ro-60/SSA,anti-Ro-52/SSA,anti-SSB,anti-Jo-1,and anti-phospholipid IgG were simultaneously detected with ELISA and protein chip technology,and numbers of the both positive,both negative and the si.
方法用酶联免疫吸附试验(EL ISA)、蛋白芯片法检测抗dsDNA、抗R o-60/SSA、抗R o-52/SSA、抗SSB、抗Jo-1、抗磷脂IgG抗体,用间接免疫荧光法(IIF)、蛋白芯片法检测抗核抗体(ANA),分别统计两种方法检测均阳性、均阴性和单阳性的标本数目,并进行评价。
3.
METHODS: Balb/c mice was immunized with pig cardiac myosin and complete freund ,s adjuvant(CFA), β 1 integrin on myocardium were analyzed by laser confocal microscopy and indirect immunofluorescence at the day 14,30,60 after the first immunization.
方法:猪肌凝蛋白皮下注射免疫Balb/c小鼠,建立慢性自身免疫性心肌炎模型,于初次免疫后14、30和60d对心肌组织进行病理学检查,同时用激光共聚焦技术和间接免疫荧光法检测整合素β1在心肌的表达变化。
5) Fluor euzymelinked immunosorbent assay
荧光酶联免疫法
6) FPIA
荧光偏振免疫法
1.
Storage and dilution requirements of cyclosporin A in whole blood sample determined by FPIA;
荧光偏振免疫法测定环孢素A血样的保存与稀释条件
2.
Influencing factors on cyclosporine A whole blood sample assay by FPIA;
荧光偏振免疫法测定环孢素A的影响因素
3.
RAM-HPLC vs.FPIA for Determination of Plasma Concentration of Carbamazepine
限进介质-HPLC法与荧光偏振免疫法测定人血浆中卡马西平浓度的比较
补充资料：精子间接免疫荧光法
精子间接免疫荧光法
诊法。免疫学检查方法之 一。用于不育症检查。①以志愿供者的精液02ml加入10～12ml生理盐水中离心，取其精子沉淀部分再置入05ml生理盐水内。②取一滴作玻璃涂片，并在空气内晾干30分钟，用纯甲醇固定30分钟，再晾干后置入pH为74的磷酸缓冲液内15分钟。③被测血清滴于上述制备的玻片上，室温下孵化30～60分钟后，用磷酸缓冲液漂洗3次，每次10分钟。④玻片上滴注 附有抗人体免疫球蛋白抗血清的异硫氰酸荧光素盐酸盐，室温下孵化30～60分钟，孵化后用磷酸缓冲液漂洗3次，每次10分钟。⑤荧光显微镜下观察结果。利用血清内的精子抗体，可使精子不同部位呈现不同免疫荧光素反应，通过与正常血清对比研究，以判断精子抗体的存在情况。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条