2) PCR combined with restriction enzyme digestion
PCR-双酶切分析
3) restriction enzyme digestion
酶切
1.
The isolated plasmid DNA was quantified by spectrophotometric measurement,and evaluated by the electrophoresis of plasmid DNA in an agarose gel,the restriction enzyme digestion,and the transfection into eukaryotic cells.
方法将含有目的质粒的菌株扩增后,运用改良后的碱裂解法进行质粒DNA小量提取,微量核酸仪测定提取质粒DNA的产量及纯度;采用琼脂糖凝胶电泳、内切酶酶切及质粒转染真核细胞鉴定提取质粒DNA的质量及转染效率。
4) cleavage
[英]['kli:vɪdʒ] [美]['klivɪdʒ]
酶切
1.
Contribution of Conservative Cleavage Motif to the Proteolytic Cleavage Within Carboxyl Terminal Domain of Rodent Muc3;
大鼠粘蛋白rMuc3酶切保守基序对其蛋白酶切的影响的研究
2.
Contribution of conservative cleavage motif to proteolytic cleavage within carboxyl terminal domain of rodent Muc3
大鼠黏蛋白rMuc3酶切保守基序对其蛋白酶切的影响
3.
Efficiencies of cleavage inboth cases were higher than those by liquid-phase dilution method.
对两种融合表达的药物蛋白尝试了固相柱上酶切的方法,即Trx-r-PA、GST-IL-11融合蛋白分别在ETI-SepharoseFF、GST-Agarose亲和柱上酶切,酶切效率均高于液相酶切法。
5) Enzyme digestion
酶切
1.
NGF by enzyme digestion was inserted into an eukaryotic expression vector,PcDNA 3 with T4 ligase.
方法 将质粒PGEM β NGF进行酶切获得 β NGF基因片段 ,用T4连接酶将其插入真核表达载体PcDNA3;以T7、P2为引物进行PCR ,论证插入片段的方向 ;经测序和酶切对插入片段进行分析和进一步鉴定。
2.
The G → A mutation created a Hha Ⅰ enzyme digestion position and the frequency was studied by asymmetric PCR-SSCP and enzyme digestion in 116 Luxi cattle and 75 Holstein cows and got the same results.
利用不对称PCR-SSCP技术,在鲁西黄牛和荷斯坦奶牛SMAD4基因的3’端非翻译区找到了1个碱基T插入突变位点和1个G→A突变位点,其中G→A突变产生了一个Hha Ⅰ酶的酶切位点。
6) Digestion
[英][daɪ'dʒestʃən] [美][daɪ'dʒɛstʃən]
酶切
1.
Preparation of highly purified vector DNA is affected by a series of factors including digestion of restriction enzyme and dephosphorylation of linearized vector DNA.
制备高质量的BAC载体DNA受到包括酶切、脱磷等诸多因素的影响。
2.
The Genomic DNA samples extracted by three methods were tested by agarose gel elctrophresis and restriction endonucleas digestion,Based on the comparative analysis of yield and quality of the DNA samples by three methods,the result showed that DNA yield of CTAB method was higher than that of SDS method and.
并通过琼脂糖凝胶电泳、限制性内切酶酶切对3种方法提取的DNA样品进行检测。
补充资料：酒化酶、醇酶、发酵酶
分子式：
CAS号：
性质：又称酒化酶、醇酶、发酵酶。从酵母中分离而得的一系列酶。不耐热。它能催化酒精发酵反应，与糖酵解有关，如磷酸化酶、醛缩酶、葡萄糖磷酸变位酶、葡萄糖6-磷酸酶等。
CAS号：
性质：又称酒化酶、醇酶、发酵酶。从酵母中分离而得的一系列酶。不耐热。它能催化酒精发酵反应，与糖酵解有关，如磷酸化酶、醛缩酶、葡萄糖磷酸变位酶、葡萄糖6-磷酸酶等。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条