1) H22 cell
H22细胞
1.
Transfection of mIL-12 gene mediated by adenoviral vector to H22 cells and its effect on the growth of H22 cells;
腺病毒介导的mIL-12基因转染H22细胞及对其生长的影响
2.
To identify the best inducing condition, we studied the expression of membrane protein HSP70 and mRNA of H22 cell at various temperature.
分别用MTT、RT-PCR、免疫荧光和FCM检测等方法观察不同热休克条件对鼠H22细胞的生存率、胞内HSP70mRNA转录水平及胞膜HSP70蛋白表达水平的改变。
2) H22 cells
H22细胞
1.
The expressions of Bcl-2 and Bax proteins in H22 cells as well as in the BALB/c H22 xenograft tumor tissues were detected using immunohistochemical method.
方法:An-nexinⅤ-FITC/PI双标记法检测苦参碱对H22细胞的早期促凋亡作用;免疫组织化学法检测苦参碱作用后H22细胞内Bc l-2、Bax两种凋亡相关蛋白的表达。
2.
Methods Treating H22 cells at different time and with different concentration of the essential oil of zanthoxylum,MTS was used to assay the cell proliferation,flow cytometry was used to assay the cell apoptosis and cell cycles.
5~8 mg/ml)分别处理H22细胞,MTS法分析细胞的增殖速度,用流式细胞仪测定细胞凋亡及细胞周期。
3.
Methods Treated with the mixture of RPMI 1640 medium and 10% mouse amniotic fluid collected respectively on gestational day 11,13,15 and 17 or RPMI 1640 medium as control,the proliferation of H22 cells was detected with MTT and the apoptosis of H22 cells with Annexin V-FITC;H22 cells were observed morphologically under electron microscope;The expressions of proliferating cell nucle.
方法用孕11、13、15 d和17 d的KM小鼠羊水-RPMI1640培养基培养H22细胞(培养液中羊水终浓度为10%),RPMI1640培养基培养的H22细胞作为对照组,采用MTT法检测细胞的增殖能力;Annexin V-FITC法检测细胞凋亡率,透射电镜观察细胞形态;S-P免疫荧光染色法经激光共聚焦显微镜分析H22细胞中增殖细胞核抗原(PCNA)和p53的表达。
3) H22
H22细胞
1.
The aim of this research was t o observe the effect of thalidomide on tumor homograft growth in mouse H22 model and to investigate the mechanisms involved and its curative possibility to hepa toma.
方法:BALB/c小鼠皮下接种H22细胞,分别从接种当天(第一给药组)和第4天(第二给药组)开始每天腹腔注射反应停50mg/kg,每天测肿瘤直径,第12天处死动物,称瘤重。
4) H22 cell line
H22细胞株
5) mouse H22 hepatocellular carcinoma cells (H22 cells)
小鼠H22肝癌细胞(H22细胞)
1.
Objective:To investigate the killing activities of tumor infiltrating lymphocytes(TILs) that were stimulated by S-DCs (dendritic cells had been sensitized by H22 cells antigen) and superantigen (SAg) staphylococcal enterotoxin A (SEA) on mouse H22 hepatocellular carcinoma cells (H22 cells) in vitro and to observe the antitumor immune capabilities of TILs stimulated by S-DCs and SEA in vivo .
目的:探讨小鼠H22肝癌细胞(H22细胞)抗原致敏树突状细胞(dendritic cell, DC)联合超抗原(superantigen,SAg)葡萄球菌肠毒素A (staphylococcalenterotoxin A,SEA)激活肿瘤浸润淋巴细胞(tumor infiltrating lymphocyte, TIL)体外对小鼠H22细胞杀伤活性的作用,并将H22全细胞抗原致敏的DC联合SEA激活TIL(H22-DC-SEA-TIL)过继免疫荷瘤小鼠,探索H22-DC-SEA-TIL在抗肿瘤免疫调节和抑瘤方面的作用,为肝癌的过继免疫治疗寻找新的有效方法和途径。
6) H22
肝癌细胞H22
1.
Application of H22 cells in study of lymphatic metastasis of tumor;
小鼠肝癌细胞H22在肿瘤淋巴道转移研究中的应用
2.
Methods:The normal L929 cells were induced by the supernatant of mice hepatocellular carcinoma cells H22 and named the induced cells as L929-H22 cells.
目的:探讨小鼠肝癌细胞H22培养上清液对鼠L929细胞细胞周期、CyclinD1、P27蛋白表达的影响。
补充资料：(22E,24R)-麦角甾烷-5,7,22-三烯-3β醇
分子式：C28H44O
分子量：396.66
CAS号：57-87-4
性质：片状结晶（乙醇）或针状结晶（乙醚）。含1.5mol结晶水者，熔点168℃。沸点250℃/1.4Pa。[α]20/D-135°（C1.2，氯仿）。
制备方法：由从葡萄糖合成该品的酵母中提取。
用途：是生产维生素D2的前体，也是生产激素类药物的中间体，可用来生产可的松。
分子量：396.66
CAS号：57-87-4
性质：片状结晶（乙醇）或针状结晶（乙醚）。含1.5mol结晶水者，熔点168℃。沸点250℃/1.4Pa。[α]20/D-135°（C1.2，氯仿）。
制备方法：由从葡萄糖合成该品的酵母中提取。
用途：是生产维生素D2的前体，也是生产激素类药物的中间体，可用来生产可的松。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条