OBJECTIVE To prepare nonviral vector of solid lipid nanoparticles carrying protamine-pDNA complex(PD-SLN),to develop a Multifunctional Envelope-type Nano Device(MEND) structure,in which the core of a plasmid DNA,condensed by a polycation,is encapsulated by a lipid envelope,then the nanoparticles characteristics,the protection ability and the DNA release property in vitro were evaluated.

The drug loading rate,encapsulation efficiency and the release rate were detected and calculated of EGF targeting nano-carrier loading c-erbB2 ASODN,and which uptake and stranded rates were exploredin SK-BR3 human breast cancer cells.