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1)  pAdBM5
pAdBM5-GFP
1.
Construction of a Novel Recombineant Adnovirus Vector pAdBM5-GFP-mAFP;
一种新的重组腺病毒载体pAdBM5-GFP-mAFP的构建
2)  GFP gene
GFP基因
1.
Salmonella choleraesuis C500 strain crp-/gfp+ mutant obtained by deleting crp gene and inserting gfp gene was constructed.
构建了猪霍乱沙门氏菌(Salmonella choleraesuis)C500株的crp缺失与插入gfp基因的crp-/gfp+缺失株。
2.
Via PCR and using plasmid pBIPG DNA as template, the GFP gene which comes from Aequorea is.
以pBIPG(携带GFP基因)的质粒DNA为模板,通过PCR技术亚克隆到了源自水母(Aequorea)大小为756bp的绿色荧光蛋白(GFP)基因,与已知序列同源率为100%。
3.
The GFP gene was transferred into the embryos of maize by the PFGE (pulsed field gel electrophoresis,PFGE) method.
利用脉冲电泳介导绿色荧光蛋白(GFP)基因导入玉米种胚;以GFP基因在种胚中瞬时表达作为外源基因导入种胚细胞的标记,分析了外源DNA浓度、电泳时间、电压、电流转换时间等脉冲电泳转化参数对种胚发芽率和外源基因导入率的影响。
3)  GFP-200 acidizing fluid
GFP-200酸液
4)  pcDNA3-GFP-PSA
pcDNA-GFP-PSA
5)  GFP mice
GFP雄鼠
1.
Results Sperm of GFP mice was no expression of green fluorescence in fluorescence microscope,while when fertilized with the KM females in vitro,the oocytes took on strong or weak expession of green fluorescent.
方法采用GFP雄鼠附睾尾部精子与KM雌鼠的卵母细胞在培养液中进行体外受精和体外培养,通过荧光显微镜观察胚胎。
6)  shRNAs
shRNAspten-GFP质粒
1.
Methods: In the OGD model with anaerobic gas mixture and deoxygenated,glucose-free extracellular solution,shRNAspten-GFP and unrelated control plasmid PshGFP transfection were done respectively.
方法:建立神经元缺糖缺氧(oxygen-glucose deprivation,OGD)损伤模型,转染shRNAspten-GFP质粒,用Western blotting检测PTEN水平,Fura2-AM法测定胞内Ca2+浓度,流式细胞术和AO/EB检测神经元凋亡。
补充资料:J基因
分子式:
CAS号:

性质: 为免疫球蛋白V区与C区之间的连接区(J区)编码的基因。

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