Study of probe substrate specificity for human cytochrome P450 isoenzymes;

Methods:Six cytochrome P450 probe substrate metabolites (including paracetamol for CYP1A2,4-hydroxytolbutamide for CYP2C9,5-hydroxyomeprazole for CYP2C19, dextrorphan for CYP2D6,6-hydroxychlorzoxazone for CYP2E1 and dehydronifedipine for CYP3A4) were measured by LC-MS/MS with positive and negative ion SRM detection.

Synthesis and spectral properties of thiazole orange compounds as biological probes;

Study of anionic dye fluorescein used as a biological probe to determine protein;

The biological probes of Fe nanowires/chiosan/α-HCG antibody were synthesized.

Using 1-(3-dimethylaminopropyl) –3-ethylcarbodiimide hydrochloride as coupling agent,as-prepared CdSe quantum dots are conjugated with Transferrin to form the bioprobe and human lung adenocarcinoma cells are successfully marked.

Phenotype analysis and probe drugs of cytochrome P450 2D6 oxidation enzyme;

Simultaneous evaluation of activity of hepatic CYP450 following administration of Fisch Fructus Forsythiae by Cocktail probe drugs;

The aim was to study the change of the rat hepatic CYP 450 content following administration of Rhizoma Curcumae Longae by cocktail probe drugs.

Establishment of a HepG_2 cell line stably expressing CYP3A22 and identification of the metabolic activity of the probe drugs

Evaluation of activity of hepatic CYP2E1 following administration of Radix et Rhizoma Rhei by probe drug in rats