In this expriment, the GFP gene was amplified by PCR from the plasmid pEGFP-N1, then it was ligated into the pLenti6/V5-D-TOPO by the TOPO cloning efficiently, quickly and directly, and constructed the recombined pLEGFP vector that could transfect the cells efficently and display protein expressing expediently.

Methods: TOPO cloning technique was selected to construct the lentivirus vector: pLenti6/V5-EGFP-MxA.

Methods PCR,TOPO-TA cloning ,molecular hybridization techniques and corresponding detecting system were used to evaluate the differential gene expression profiles of GlcNAc6ST,GalT and GnT in different tumor cell lines.

Methods Mainly by means of PCR, TOPO-TA cloning and molecular hybridization techniques.

Clone origin of dysplastic and non-dysplastic bone marrow hematopoitic cells in myeloid dysplasia patient with 5q-complex karyotype;

Clone and expression of the nucleoprotein gene from type A influenza virus separated in Hubei in 2006;

Clone of the hTERT Promoter and Its Specific Transcriptional Activity in Human Gastric Caner Cells;

Analysis of Cloning and Phylogenetic of 16S rRNA Gene from a Naphthalene Degrading Bacterium NAP2-2;

Screening of Uricase Producing Strains,Cloning and Expression of Uricase Gene;

Cloning,Expression and Initial Identification of Extracellular Region Gene Fragment of Herpes Simplex Viruses l;

Molecular Cloning and Sequence Analysis of Ovine Regakine-1;

Molecular Cloning and Sequence Analysis of Porcine Musclin;

This paper aims at molecular cloning and functional elucidation of a putative STK sequence in Spirulina platensis.

Restriction enzyme digestion and AT clone were employed to rapidly screen out the recons of PA segments for the preparation of the gene chip probes.