半定量反转录-聚合酶链反应,RT-PCR,在线英语词典,英文翻译,专业英语

1) RT-PCR

半定量反转录-聚合酶链反应

Methods The expression of BCRP gene in 54 AML and multidrug resistence transporter P-glycoprotein(MDR1) gene in 8 AML patients were examined by semi-quantitative reverse transcriptase-polymerase chain reaction(RT-PCR).

方法应用半定量反转录-聚合酶链反应(RT-PCR),以MCF7乳腺癌细胞株、HL60白血病细胞株分别作为BCRP及MDR1 mRNA表达的参照标准,以β肌动蛋白(-βactin)为内对照,检测了54例AML患者BCRP mRNA的表达,其中8例患者测定MDR1 mRNA的表达,初步探讨BCRP表达在AML中的临床意义。

2) semi-quantitive RT-PCR

半定量反转录聚合酶链反应

An insulin-like growth factors-Ⅰ gene(IGFⅠ) from geese was cloned and its expression dynamics in tissues was examined by semi-quantitive RT-PCR using β-actin as an internal standard.

利用半定量反转录聚合酶链反应(RT-PCR)克隆了黑龙江籽鹅IGF-Ⅰ基因,并检测了生长期间该基因mRNA在各组织中的表达量变化。

3) semidefinite

半定

4) Semi-definite and Semi-quantitative

半定性与半定量

5) half fixed-point

半定点

,the adjusting mining method with half fixed-point is applied.

淮南矿业集团公司潘三煤矿根据1542(3)工作面收作需甩采70 m的实际,采用半定点甩采的方法,较好地控制了链板机的下滑,加快了推进度,提高了煤炭资源回收率。

6) semi-quantitative

半定量

Based on the relationship established between the reaction degree of mineral admixture and the consumption of Ca(OH)_2,and the equilibrium calculation theory of cement hydration,the decoupling method of Ca(OH)_2 content in hydration products of blended cement paste was proposed and used to semi-quantitatively calculate the content of C-S-H gel.

基于掺合料反应程度与Ca(OH)2消耗量之间关系的建立,结合水泥水化平衡计算理论,提出混合水泥水化产物中Ca(OH)2量的解耦方法并将其用于混合水泥水化产物C-S-H凝胶半定量计算。

In this paper,a modified method based on Olson's water absorption method has been proposed and a semi-quantitative study on C-S-H .

在Olson水吸附法的基础上,针对其存在的问题,提出了改进水吸附法,然后用改进水吸附法对水泥中的C-S-H凝胶进行了半定量分析。

Methods: Using reverse transcription-polymerase chain reaction(RT-PCR),cavies lung tissue Eotaxin mRNA expression were given semi-quantitative analysis.

方法:采用逆转录-聚合酶链式反应(RT-PCR)法半定量分析哮喘豚鼠肺组织嗜酸性粒细胞趋化因子(eotaxin)mRNA表达水平的变化。

参考词条

半定量RTPCR 半定松弛半定规化半定量化正半定半定优化半定松驰半定制半定量法半定量 PCR 半定制ASIC 半定矩阵微细浸染型层控金矿床芒果素

补充资料：聚合酶链反应

聚合酶链反应
polymerase chain reaction,PCR

是用特异性寡核苷酸引物在DNA聚合酶的作用下对靶DNA序列进行大量扩增的分子生物学方法。PCR技术中以在短时间内把极其微量的特定DNA(或RNA）片断大量增扩，达到用常规方法就可以检测的水平。临床用来确定一些疾病的病原体，因其假阳性率较高，故应谨慎使用。

说明：补充资料仅用于学习参考，请勿用于其它任何用途。

首页 \| 翻译 \| 影视 \| 论坛 \| 索引半定量PCR 半定规划半定初态半定性半定向型半定量-PCR

1) RT-PCR 半定量反转录-聚合酶链反应 1. Methods The expression of BCRP gene in 54 AML and multidrug resistence transporter P-glycoprotein(MDR1) gene in 8 AML patients were examined by semi-quantitative reverse transcriptase-polymerase chain reaction(RT-PCR). 方法应用半定量反转录-聚合酶链反应(RT-PCR),以MCF7乳腺癌细胞株、HL60白血病细胞株分别作为BCRP及MDR1 mRNA表达的参照标准,以β肌动蛋白(-βactin)为内对照,检测了54例AML患者BCRP mRNA的表达,其中8例患者测定MDR1 mRNA的表达,初步探讨BCRP表达在AML中的临床意义。 2) semi-quantitive RT-PCR 半定量反转录聚合酶链反应 1. An insulin-like growth factors-Ⅰ gene(IGFⅠ) from geese was cloned and its expression dynamics in tissues was examined by semi-quantitive RT-PCR using β-actin as an internal standard. 利用半定量反转录聚合酶链反应(RT-PCR)克隆了黑龙江籽鹅IGF-Ⅰ基因,并检测了生长期间该基因mRNA在各组织中的表达量变化。 3) semidefinite 半定 4) Semi-definite and Semi-quantitative 半定性与半定量 5) half fixed-point 半定点 1. ,the adjusting mining method with half fixed-point is applied. 淮南矿业集团公司潘三煤矿根据1542(3)工作面收作需甩采70 m的实际,采用半定点甩采的方法,较好地控制了链板机的下滑,加快了推进度,提高了煤炭资源回收率。 6) semi-quantitative 半定量 1. Based on the relationship established between the reaction degree of mineral admixture and the consumption of Ca(OH)_2,and the equilibrium calculation theory of cement hydration,the decoupling method of Ca(OH)_2 content in hydration products of blended cement paste was proposed and used to semi-quantitatively calculate the content of C-S-H gel. 基于掺合料反应程度与Ca(OH)2消耗量之间关系的建立,结合水泥水化平衡计算理论,提出混合水泥水化产物中Ca(OH)2量的解耦方法并将其用于混合水泥水化产物C-S-H凝胶半定量计算。 2. In this paper,a modified method based on Olson's water absorption method has been proposed and a semi-quantitative study on C-S-H . 在Olson水吸附法的基础上,针对其存在的问题,提出了改进水吸附法,然后用改进水吸附法对水泥中的C-S-H凝胶进行了半定量分析。 3. Methods: Using reverse transcription-polymerase chain reaction(RT-PCR),cavies lung tissue Eotaxin mRNA expression were given semi-quantitative analysis. 方法:采用逆转录-聚合酶链式反应(RT-PCR)法半定量分析哮喘豚鼠肺组织嗜酸性粒细胞趋化因子(eotaxin)mRNA表达水平的变化。参考词条半定量RTPCR 半定松弛半定规化半定量化正半定半定优化半定松驰半定制半定量法半定量 PCR 半定制ASIC 半定矩阵微细浸染型层控金矿床芒果素补充资料：聚合酶链反应聚合酶链反应 polymerase chain reaction,PCR 是用特异性寡核苷酸引物在DNA聚合酶的作用下对靶DNA序列进行大量扩增的分子生物学方法。PCR技术中以在短时间内把极其微量的特定DNA(或RNA）片断大量增扩，达到用常规方法就可以检测的水平。临床用来确定一些疾病的病原体，因其假阳性率较高，故应谨慎使用。 说明：补充资料仅用于学习参考，请勿用于其它任何用途。
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