1) packaging cell line PT67
包装细胞株
1.
The recombinant plasmid was transferred into packaging cell line PT67 with Lipofectamine 2000TM and the efficient virus produci.
目的用pLXSN反转录病毒载体构建介导β-分泌酶底物肽(BACEsp)基因表达的pLXSN-BACEsp反转录病毒载体,并建立高效、稳定表达BACEsp的包装细胞株。
2) package cell
包装细胞
1.
The culture of retrovirus package cell and the package process;
逆转录病毒载体包装细胞的培养及包装过程(英文)
2.
Construction of mIL-12 recombinant retrovirus vector and establishment of viral package cell line;
mIL-12重组逆转录病毒载体的构建及包装细胞系的建立
3) packing cell
包装细胞
1.
Then,two packing cell lines (ecotrophic ψ2 and amphotrophic PA317) were transfected,and the positive cellular clones were screened by G418.
将该目的基因插入LXSN逆转录病毒载体 ,并转染大肠杆菌DH5α中扩增 ,再感染 ψ2 (ecotropic )和PA317(amphotropic )两种包装细胞 ,经G4 18筛选后获得表达IL 2 3分子的PA317阳性细胞克隆。
4) packaging cell
包装细胞
1.
AIM To optimize the methods of manufacturing retrovirus containing supernatant of PA317 packaging cell line for the use in the retroviral mediated gene transfer.
目的 探讨产生重组逆转录病毒包装细胞系建立及其病毒滴度 (以 cfu表示 )的影响因素 。
2.
bjective:To construct the hIL-2 retroviral vectors and the packaging cells.
目的：构建人白细胞介素２（ｈＩＬ－２）逆转录病毒载体及包装细胞。
5) Packaging cell line
包装细胞系
1.
Stable packaging cell lines were developed, which make production of lentivirus vector convenient.
经过结构优化的慢病毒载体已经用于转基因动物生产和基因治疗研究,而稳定包装细胞系的建立使得慢病毒的生产更为简便。
2.
Conclusion A stable virus producing packaging cell line with high titer was established successfully.
结论成功建立了pLXSN-CAT-MAR的包装细胞系。
6) packaging cell lines
包装细胞系
1.
A replication-defective retrovirus vector containing the human β-globin gene and 36base-pair HS2 enhancer( N2AβE36) was constructed and transfered into ψ-2 and PA317 packaging cell lines,Titers of amphotropic recombinant retrovirus ranged from 103 to 104 CFU/ml.
将此反转录病毒重组体转染ψ－２和ＰＡ３１７包装细胞系，经Ｇ４１８筛选后，分离出完整整合的并能产生较高病毒滴度的产病毒细胞系。
补充资料：细胞株
分子式：
CAS号：
性质：用单细胞分离培养法或克隆形成法从原代培养或从细胞系所选出的细胞群，称细胞株。一个细胞株应具有特定的生物学性质和标记并持续存在。
CAS号：
性质：用单细胞分离培养法或克隆形成法从原代培养或从细胞系所选出的细胞群，称细胞株。一个细胞株应具有特定的生物学性质和标记并持续存在。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条