1) Hepatocellular carcinoma cell line
肝癌细胞株
1.
And then the retrovirus containing human ICE cDNA generated by these PA317 cells were used to transfect human hepatocellular carcinoma cell line HepG2.
方法 :应用电穿孔法将构建成功含有目的基因的逆转录病毒载体 pLXSN hICE导入包装细胞系PA317,筛选G4 18抗性克隆 ,并将其病毒上清转入肝癌细胞株HepG2 ,DNA提取、电泳观察 ;采用3 H TDR掺入法观察、分析化疗药物卡铂对肝癌细胞株SMMC772 1及转入相应目的基因后的SMMC772 1 ICE ,SMMC772 1 反义hICE ,SMMC772 1 neo细胞株体外增殖的影响。
2.
The plasmid pCI/mdr1 was transferred to human hepatocellular carcinoma cell line HepG2 by liposome.
方法构建表达mdr1cDNA全长序列的真核表达载体PCI/mdr1,利用脂质体将mdr1cDNA转染入人肝癌细胞株HepG2细胞,通过阿霉素短暂诱导和G418筛选转染阳性细胞,筛选出稳定表达mdr1及P-gp蛋白的细胞株HepG2/R。
2) Hepatoma cell line
肝癌细胞株
1.
Effect of arsenic trioxide to human hepatoma cell line BEL-7402 cultured in vitro;
亚砷酸体外对人肝癌细胞株BEL-7402影响的初步研究
2.
Methods:The growth inhibitory effect of emodin on Human Hepatoma cell line smmc-7721 was observed by using MTT assay,cell apoptosis was analyzed using flow cytometry,agrose gel electrophoresis of DNA and fluorescent microscopy.
方法:肝癌细胞smmc-7721经emodin(大黄素)处理后,用MTT法观察emodin对肝癌细胞株smmc-7721细胞增殖的抑制作用,荧光显微镜、DNA凝胶电泳、流式细胞仪分析诱导凋亡作用,采用western blot检测p53、p21蛋白水平变化,采用细胞免疫组化方法检测Fas的表达变化。
3.
Objective To study the apoptosis induced by emodin in human hepatoma cell line smmc-7721 and to clarify its molecular mechanism.
方法 肝癌细胞smmc-7721经药物处理后,用四甲基偶氮唑蓝(MTT)比色还原法观察大黄素对肝癌细胞株smmc-7721细胞生长的抑制作用;普通显微镜、荧光显微镜观察细胞形态学的变化、DNA凝胶电泳、流式细胞仪(FCM)分析诱导凋亡作用;采用免疫印迹法(Western blot)检测p53、p21蛋白水平变化,采用细胞免疫化学(immunocytochemistry)方法检测Fas的表达变化。
3) hepatocarcinoma cell
肝癌细胞株
1.
To apply the small interfering RNAs targeting survivin to inhibit expression of endogenous survivin gene in human hepatocarcinoma cell SMMC-7721, the recombinant plasmid pshRNA-survivins were transfectted into SMMC-7721.
应用RNA干扰技术(RNAi)研究针对凋亡抑制因子survivin的siRNA抑制肝癌细胞株内源survivin基因的表达。
4) Liver cancer cell line
肝癌细胞株
1.
Effect of Survivin-antisense on Liver Cancer Cell Line SMMC-7721 in Inducing Apoptosis and Increasing its Sensitivity to Antineoplastic Agents;
生存素反义核酸诱导肝癌细胞株SMMC-7721凋亡和增加其对抗肿瘤药物敏感性的研究
5) hepatoma cell
肝癌细胞株
1.
Methods: Hep-G 2 human hepatoma cells made by transfection with expressible MT1M gene, and the cell cycle was detected by flow cytometry, and the signaling pathway was measured by dual luciferase assay in Hep-G 2 cells.
方法 :构建表达MT1M基因的真核质粒 ,转染Hep G2 肝癌细胞株 ,流式细胞术检测该基因对细胞周期的影响 ,通过双荧光素酶报告系统检测该基因对细胞信号通路的影响。
6) hepatocellular carcinoma cell lines
肝细胞癌细胞株
1.
Over-expression of p27~(kip1) affects cell cycle of hepatocellular carcinoma cell lines;
p27~(kip1)过表达对肝细胞癌细胞株细胞周期的影响
补充资料：细胞株
分子式：
CAS号：
性质：用单细胞分离培养法或克隆形成法从原代培养或从细胞系所选出的细胞群，称细胞株。一个细胞株应具有特定的生物学性质和标记并持续存在。
CAS号：
性质：用单细胞分离培养法或克隆形成法从原代培养或从细胞系所选出的细胞群，称细胞株。一个细胞株应具有特定的生物学性质和标记并持续存在。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条