1) Whole-mount in situ hybridization
整体原位杂交
1.
Expression of rnf141 gene in the embryos of different developmental stages and tissues of adult fish were examined by whole-mount in situ hybridization and multiple-tissue RT-PCR technique.
方法:采用生物信息学方法分析斑马鱼rnf141基因结构特征;运用胚胎整体原位杂交和多组织RT-PCR技术分别检测斑马鱼胚胎发育不同阶段和成体各组织rnf141基因表达情况。
2.
The results obtained were consistent with those in paper previously reported,suggesting that the DNA probe produced by PCR is suitable for whole-mount in situ hybridization on Xenopus embryos under specific co.
整体原位杂交 (Whole mountinsituhybridization)用于基因表达定位和表达分布模式的研究 ,已经成为一种非常重要的手段 。
3.
Objective To observe the expression of Shh gene in the maxillofacial region of mouse embryo by whole-mount in situ hybridization.
目的应用整体原位杂交技术探讨Shh 基因在小鼠胚胎颁面部正常发育中的表达。
2) whole mount in situ hybridization
整体原位杂交
1.
Methods We collected all the phases of zebrafish embryos to detect the expression pattern of Mef2c in the somite and the heart during zebrafish embryogenesis by whole mount in situ hybridization.
方法收集不同发育时期的AB野生型斑马鱼胚胎,采用整体原位杂交技术检测Mef2c在骨骼肌和心肌的转录情况。
3) whole mount flurescence in situ hybridization (WFISH)
整体荧光原位杂交(WFISH)
4) WFISH
整体荧光原位杂交
5) whole mount in situ hybridization
整胚原位杂交
6) chromosome in situ hybridization
染色体原位杂交
1.
The chromosome in situ hybridization is an important part in modern biotechnology.
染色体原位杂交技术是现代生物技术的重要组成部分。
2.
The technique of chromosome in situ hybridization (CISH) has been extensively applied to genetics and breeding of higher plants.
染色体原位杂交技术近年发展较快,已广泛应用于植物遗传育种实践。
补充资料：荧光原位杂交
荧光原位杂交
是以荧光素标记取代核素标记而形成的一种新的原位杂交方法。利用已知碱基序列的非核素标记的核酸探针，依据碱基配对原理，通过免疫细胞化学检测体系，在组织切片、细胞间期核或染色体等标本上，进行DNA定性、定位及定量分析。本法快速、安全、灵敏度高，特异性强。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条