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1)  Modified Chelex-100 method
改良Chelex-100法
2)  Chelex-100 method
Chelex-100法
1.
Objective To study the relation between the quantity of DNA extracted by Chelex-100 method and multiplex STRs analysis.
目的研究Chelex-100法提取的生物检材DNA用量与复合STR分型成功率的关系。
2.
Improved Chelex-100 method and conventional CTAB method were applied to extract total DNA from transgenic roundup ready soybean.
以转基因抗草甘膦大豆为研究材料,分别采用改良Chelex-100法和常规CTAB法提取基因组DNA,以提取DNA的浓度和纯度,同时以PCR扩增大豆的内源基因(lectin)及外源特异性序列(CaMV35S,nos,Cp4-epsps)的效果对两种方法进行比较和评价。
3.
Methods 76 DNA samples of epithelial cells extracted by Chelex-100 method were quantitated by ABI 7500 Real Time System and typed with Identifiler system in ABI 3130 Genetic Analyzer.
方法对76份包括帽子、眼镜、牙刷、剃须刀、梳子、口香糖、羊水在内的7种脱落细胞检材采用Chelex-100法提取DNA,在ABI7500型荧光定量PCR仪上进行定量,同时用Identifiler复合扩增系统扩增,在ABI3130遗传分析仪上进行STR分型。
3)  Chelex-100
Chelex-100法
1.
Methods: DNA of subgingival plaque was extracted by two differedt kinds of methods,which were UNIQ-10 Bateria Kit and Chelex-100 assay .
方法:采用UNIQ-10柱式细菌基因组DNA抽提试剂盒法和Chelex-100法获取患者龈下菌斑DNA,比较作为PCR扩增模板检测牙龈卟啉单胞菌感染位点的检出率差异,并对2种方法进行比较。
2.
Methods DNA fragments were extracted from costal cartilages of different colour of 30 corrupt corpses using Chelex-100 and Phenol-Chloroform methods.
方法利用Chelex-100法和酚-氯仿法,分别对30例不同色泽的腐败尸体肋软骨进行DNA提取,STR复合扩增,ABI3100型基因分析仪对扩增产物进行检测。
4)  Chelex-100 resin method
Chelex-100树脂法
5)  mini system Chelex-100 method
小体积Chelex-100法
1.
Methods One hundred DNA samples of oral epithelial cells extracted by mini system Chelex-100 method were quantitated by ABI 7500 Real Time System and were then typed with IdentifilerTM system in ABI 3130 Genetic Analyzer.
方法对不同载体上的100份微量口腔脱落细胞检材采用小体积Chelex-100法提取DNA,在ABI7500型荧光定量PCR仪上进行定量,同时用IdentifilerTM复合扩增系统扩增,在ABI3130遗传分析仪上进行STR分型。
6)  chelex-100-boiling
chelex-100裂解液加热煮沸
1.
And confirm the sensitivity of chelex-100-boiling in these two bacteria.
结论:chelex-100裂解液加热煮沸法抽提细菌基因组DNA操作简便、省时、经济,为血液标本细菌基因检测在临床上的大规模应用奠定基础。
补充资料:改良型曝气法
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CAS号:

性质:废水活性污泥法的一种变型。流程与常规活性污泥法相同,但采用缩短曝气时间(1.5~3h),并减少混合液中悬浮固体的含量(200≈500mg/L MLSS)。其污泥沉降性一般较差,出水中悬浮固体的含量较高。此法可用作中等程度的处理,只需设置格栅和沉砂池作为预处理,不需设初次沉淀池。其污泥回流率小(5%~15%),可减少回流污泥动力消耗。

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